PMID- 25361662
OWN - NLM
STAT- MEDLINE
DCOM- 20150624
LR  - 20211021
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 1203
DP  - 2015
TI  - Sample preparation for 3D SIMS chemical imaging of cells.
PG  - 9-19
LID - 10.1007/978-1-4939-1357-2_2 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is an emerging 
      technique for the characterization of biological systems. With the development of 
      novel ion sources such as cluster ion beams, ionization efficiency has been 
      increased, allowing for greater amounts of information to be obtained from the 
      sample of interest. This enables the plotting of the distribution of chemical 
      compounds against position with submicrometer resolution, yielding a chemical map 
      of the material. In addition, by combining imaging with molecular depth 
      profiling, a complete 3-dimensional rendering of the object is possible. The 
      study of single biological cells presents significant challenges due to the 
      fundamental complexity associated with any biological material. Sample 
      preparation is of critical importance in controlling this complexity, owing to 
      the fragile nature of biological cells and to the need to characterize them in 
      their native state, free of chemical or physical changes. Here, we describe the 
      four most widely used sample preparation methods for cellular imaging using 
      ToF-SIMS, and provide guidance for data collection and analysis procedures.
FAU - Winograd, Nicholas
AU  - Winograd N
AD  - Department of Chemistry, Pennsylvania State University, 209 Chemistry Building, 
      University Park, PA, 16802, USA, nxw@psu.edu.
FAU - Bloom, Anna
AU  - Bloom A
LA  - eng
GR  - P41 GM103391/GM/NIGMS NIH HHS/United States
GR  - P41 RR031461/RR/NCRR NIH HHS/United States
GR  - 8 P41 GM103391/GM/NIGMS NIH HHS/United States
GR  - 5P41RR031461/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 1HG84L3525 (Formaldehyde)
RN  - T3C89M417N (Glutaral)
SB  - IM
MH  - Analytic Sample Preparation Methods/*methods
MH  - Formaldehyde
MH  - Freeze Drying
MH  - Glutaral
MH  - HeLa Cells
MH  - Humans
MH  - Imaging, Three-Dimensional/*methods
MH  - Molecular Imaging/*methods
MH  - Paraffin Embedding
MH  - Spectrometry, Mass, Secondary Ion/*methods
MH  - Tissue Fixation
PMC - PMC5642907
MID - NIHMS910622
EDAT- 2014/11/02 06:00
MHDA- 2015/06/25 06:00
PMCR- 2017/10/16
CRDT- 2014/11/02 06:00
PHST- 2014/11/02 06:00 [entrez]
PHST- 2014/11/02 06:00 [pubmed]
PHST- 2015/06/25 06:00 [medline]
PHST- 2017/10/16 00:00 [pmc-release]
AID - 10.1007/978-1-4939-1357-2_2 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1203:9-19. doi: 10.1007/978-1-4939-1357-2_2.