PMID- 25361819
OWN - NLM
STAT- MEDLINE
DCOM- 20150930
LR  - 20150203
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 160
IP  - 2
DP  - 2015 Feb
TI  - Full-length genome sequence analysis of a Hungarian porcine reproductive and 
      respiratory syndrome virus isolated from a pig with severe respiratory disease.
PG  - 417-22
LID - 10.1007/s00705-014-2265-2 [doi]
AB  - Here, we report the isolation of a type 1 porcine reproductive and respiratory 
      syndrome virus (PRRSV) strain from a clinical outbreak of severe respiratory 
      problems and high fever. Next-generation sequencing was used to determine the 
      complete genome sequence of the isolate (9625/2012). The virus belongs to a new 
      branch within subtype 1, clade D, and shows the highest similarity to PRRSV 
      Olot/1991 and to the Amervac vaccine strain. Mutation analysis of 9625/2012 
      revealed no evidence of recombination but did show a high proportion of amino 
      acid substitutions in the putative neutralizing epitopes, suggesting an important 
      role of selective immune pressure in the evolution of PRRSV 9625/2012.
FAU - Balint, Adam
AU  - Balint A
AD  - National Food Chain Safety Office Veterinary Diagnostic Directorate, Tabornok u. 
      2, 1149, Budapest, Hungary, balintad@nebih.gov.hu.
FAU - Balka, Gyula
AU  - Balka G
FAU - Horvath, Peter
AU  - Horvath P
FAU - Kecskemeti, Sandor
AU  - Kecskemeti S
FAU - Dan, Adam
AU  - Dan A
FAU - Farsang, Attila
AU  - Farsang A
FAU - Szeredi, Levente
AU  - Szeredi L
FAU - Banyai, Krisztian
AU  - Banyai K
FAU - Bartha, Daniel
AU  - Bartha D
FAU - Olasz, Ferenc
AU  - Olasz F
FAU - Belak, Sandor
AU  - Belak S
FAU - Zadori, Zoltan
AU  - Zadori Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141102
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Animals
MH  - Base Sequence
MH  - Coinfection
MH  - Disease Outbreaks/*veterinary
MH  - Genome, Viral/*genetics
MH  - High-Throughput Nucleotide Sequencing
MH  - Hungary/epidemiology
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Mycoplasma hyopneumoniae
MH  - Pneumonia of Swine, Mycoplasmal/pathology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology/pathology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/isolation & purification
MH  - RNA, Viral/genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, RNA/veterinary
MH  - Swine
EDAT- 2014/11/02 06:00
MHDA- 2015/10/01 06:00
CRDT- 2014/11/02 06:00
PHST- 2014/05/08 00:00 [received]
PHST- 2014/10/23 00:00 [accepted]
PHST- 2014/11/02 06:00 [entrez]
PHST- 2014/11/02 06:00 [pubmed]
PHST- 2015/10/01 06:00 [medline]
AID - 10.1007/s00705-014-2265-2 [doi]
PST - ppublish
SO  - Arch Virol. 2015 Feb;160(2):417-22. doi: 10.1007/s00705-014-2265-2. Epub 2014 Nov 
      2.