PMID- 25363062
OWN - NLM
STAT- MEDLINE
DCOM- 20150821
LR  - 20221207
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 118
IP  - 1
DP  - 2015 Jan
TI  - Probiotic supplementation in systemic nickel allergy syndrome patients: study of 
      its effects on lactic acid bacteria population and on clinical symptoms.
PG  - 202-11
LID - 10.1111/jam.12685 [doi]
AB  - AIMS: The study aimed to evaluate the effects of probiotic Lactobacillus reuteri 
      DSM 17938 strain supplementation in patients suffering from systemic nickel 
      allergy syndrome, in terms of modulation of faecal LAB population linked to a 
      reduction of GI and cutaneous symptoms and to an increase of patient's quality of 
      life. METHODS AND RESULTS: A preliminary double-blind randomized 
      placebo-controlled study was planned and a culture-independent method based on 
      denaturing gradient gel electrophoresis (DGGE) analysis coupled to the 16S rRNA 
      gene sequencing was applied to investigate on the dynamics of faecal LAB 
      communities before and during a low-Ni diet, supplemented with the probiotic 
      strain. Moreover, the severity and the frequency of GI and cutaneous reactions as 
      well as patient's clinical condition perception (VAS scores) were estimated by 
      statistical analysis. PCR-DGGE fingerprinting obtained using LAB-specific primers 
      revealed significant shift in faecal community with an increase in biodiversity 
      in patients supplemented with probiotic Lact. reuteri strain. In addition, GI 
      reactions such as symptoms related to meals and type of stools significantly 
      improved only in patients treated with Lact. reuteri, while severity and 
      frequency of cutaneous symptoms (urticaria, itch and eczema) and recurrent 
      abdominal pain (RAP) as well as VAS scores statistically decreased in both 
      groups. CONCLUSIONS: Our preliminary findings suggest that probiotic 
      Lact. reuteri could be a useful supplementation during a low-Ni diet of patients 
      with SNAS, to increase LAB population diversity, which could contribute to 
      restore the intestinal homoeostasis conditions. SIGNIFICANCE AND IMPACT OF THE 
      STUDY: To date, no information is available on probiotics application and on 
      their effects, in terms of intestinal microbiota modulation, on patients 
      suffering from SNAS. Therefore, the identification of dominant LAB community and 
      the study of its shift during the probiotic supplementation could enhance the 
      knowledge of the SNAS syndrome.
CI  - (c) 2014 The Society for Applied Microbiology.
FAU - Randazzo, C L
AU  - Randazzo CL
AD  - Department of Agri-food and Environmental Systems Management, University of 
      Catania, Catania, Italy.
FAU - Pino, A
AU  - Pino A
FAU - Ricciardi, L
AU  - Ricciardi L
FAU - Romano, C
AU  - Romano C
FAU - Comito, D
AU  - Comito D
FAU - Arena, E
AU  - Arena E
FAU - Saitta, S
AU  - Saitta S
FAU - Caggia, C
AU  - Caggia C
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20141130
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7OV03QG267 (Nickel)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Double-Blind Method
MH  - Feces/microbiology
MH  - Female
MH  - Humans
MH  - Hypersensitivity/diagnosis/etiology/microbiology/*therapy
MH  - Lactobacillus/genetics/*isolation & purification
MH  - *Limosilactobacillus reuteri
MH  - Middle Aged
MH  - Nickel/*toxicity
MH  - Polymerase Chain Reaction
MH  - Probiotics/*therapeutic use
MH  - RNA, Ribosomal, 16S
MH  - Sequence Analysis, DNA
MH  - Young Adult
OTO - NOTNLM
OT  - Lact. reuteri
OT  - PCR-DGGE
OT  - SNAS
OT  - clinical outcomes
OT  - faecal samples
OT  - low-nickel diet
EDAT- 2014/11/05 06:00
MHDA- 2015/08/22 06:00
CRDT- 2014/11/04 06:00
PHST- 2014/09/04 00:00 [received]
PHST- 2014/10/21 00:00 [revised]
PHST- 2014/10/27 00:00 [accepted]
PHST- 2014/11/04 06:00 [entrez]
PHST- 2014/11/05 06:00 [pubmed]
PHST- 2015/08/22 06:00 [medline]
AID - 10.1111/jam.12685 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2015 Jan;118(1):202-11. doi: 10.1111/jam.12685. Epub 2014 Nov 
      30.