PMID- 25364581
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141103
LR  - 20200930
IS  - 2095-3941 (Print)
IS  - 2095-3941 (Linking)
VI  - 11
IP  - 3
DP  - 2014 Sep
TI  - The gene expression patterns of BMPR2, EP300, TGFbeta2, and TNFAIP3 in B-Lymphoma 
      cells.
PG  - 202-7
LID - 10.7497/j.issn.2095-3941.2014.03.006 [doi]
AB  - OBJECTIVE: The results of a previous study showed that a clear dysregulation was 
      evident in the global gene expression of the BCL11A-suppressed B-lymphoma cells. 
      In this study, the bone morphogenetic protein receptor, type II (BMPR2), E1A 
      binding protein p300 (EP300), transforming growth factor-beta2 (TGFbeta2), and tumor 
      necrosis factor, and alpha-induced protein 3 (TNFAIP3) gene expression patterns 
      in B-cell malignancies were studied. METHODS: The relative expression levels of 
      BMPR2, EP300, TGFbeta2, and TNFAIP3 mRNA in B-lymphoma cell lines, myeloid cell 
      lines, as well as in cells from healthy volunteers, were determined by real-time 
      quantitative reverse transcript-polymerase chain reaction (qRT-PCR) with SYBR 
      Green Dye. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as 
      reference. RESULTS: The expression level of TGFbeta2 mRNA in B-lymphoma cell lines 
      was significantly higher than those in the cells from the healthy control 
      (P<0.05). However, the expression level of TNFAIP3 mRNA in B-malignant cells was 
      significantly lower than that of the healthy control (P<0.05). The expression 
      levels of BMPR2 and EP300 mRNA showed no significant difference between 
      B-malignant cell lines and the healthy group (P>0.05). In B-lymphoma cell lines, 
      correlation analyses revealed that the expression of BMPR2 and TNFAIP3 (r=0.882, 
      P=0.04) had significant positive relation. The expression levels of BMPR2, EP300, 
      and TNFAIP3 mRNA in cell lines from myeloid leukemia were significantly lower 
      than those in the cells from the healthy control (P<0.05). The expression levels 
      of TGFbeta2 mRNA showed no significant difference between myeloid leukemia cell 
      lines and the healthy control or B-malignant cell lines (P>0.05). The expression 
      levels of BMPR2, EP300, and TNFAIP3 mRNA in B-lymphoma cells were significantly 
      higher than those of the myeloid leukemia cells (P<0.05). CONCLUSION: Different 
      expression patterns of BMPR2, EP300, TGFbeta2, and TNFAIP3 genes in B-lymphoma cells 
      exist.
FAU - He, Dong-Mei
AU  - He DM
AD  - 1 Institute of Hematology, Medical College, 2 Key Laboratory for Regenerative 
      Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
FAU - Wu, Hong
AU  - Wu H
AD  - 1 Institute of Hematology, Medical College, 2 Key Laboratory for Regenerative 
      Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
FAU - Wu, Xiu-Li
AU  - Wu XL
AD  - 1 Institute of Hematology, Medical College, 2 Key Laboratory for Regenerative 
      Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
FAU - Ding, Li
AU  - Ding L
AD  - 1 Institute of Hematology, Medical College, 2 Key Laboratory for Regenerative 
      Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
FAU - Xu, Ling
AU  - Xu L
AD  - 1 Institute of Hematology, Medical College, 2 Key Laboratory for Regenerative 
      Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
FAU - Li, Yang-Qiu
AU  - Li YQ
AD  - 1 Institute of Hematology, Medical College, 2 Key Laboratory for Regenerative 
      Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Cancer Biol Med
JT  - Cancer biology & medicine
JID - 101588850
PMC - PMC4197423
OTO - NOTNLM
OT  - B-lymphoma cells
OT  - Bone morphogenetic protein receptor, type II (BMPR2)
OT  - E1A binding protein p300 (EP300)
OT  - myeloid leukemia cells
OT  - quantitative reverse transcription polymerase chain reaction (qRT-PCR)
OT  - transforming growth factor-beta2 (TGFbeta2)
OT  - tumor necrosis factor, and alpha-induced protein 3 (TNFAIP3)
COIS- No potential conflicts of interest are disclosed.
EDAT- 2014/11/05 06:00
MHDA- 2014/11/05 06:01
PMCR- 2014/09/01
CRDT- 2014/11/04 06:00
PHST- 2014/04/09 00:00 [received]
PHST- 2014/06/02 00:00 [accepted]
PHST- 2014/11/04 06:00 [entrez]
PHST- 2014/11/05 06:00 [pubmed]
PHST- 2014/11/05 06:01 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - cbm-11-03-202 [pii]
AID - 10.7497/j.issn.2095-3941.2014.03.006 [doi]
PST - ppublish
SO  - Cancer Biol Med. 2014 Sep;11(3):202-7. doi: 10.7497/j.issn.2095-3941.2014.03.006.