PMID- 25364831
OWN - NLM
STAT- MEDLINE
DCOM- 20150810
LR  - 20240313
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 11
DP  - 2014
TI  - Elevation of peripheral BDNF promoter methylation links to the risk of 
      Alzheimer's disease.
PG  - e110773
LID - 10.1371/journal.pone.0110773 [doi]
LID - e110773
AB  - Brain derived neurotrophic factor (BDNF) has been known to play an important role 
      in various mental disorders or diseases such as Alzheimer's disease (AD). The aim 
      of our study was to assess whether BDNF promoter methylation in peripheral blood 
      was able to predict the risk of AD. A total of 44 AD patients and 62 age- and 
      gender-matched controls were recruited in the current case-control study. Using 
      the bisulphite pyrosequencing technology, we evaluated four CpG sites in the 
      promoter of the BDNF. Our results showed that BDNF methylation was significantly 
      higher in AD cases than in the controls (CpG1: p = 10.021; CpG2: p = 0.002; CpG3: 
      p = 0.007; CpG4: p = 0.005; average methylation: p = 0.004). In addition, BDNF 
      promoter methylation was shown to be significantly correlated with the levels of 
      alkaline phosphatase (ALP), glucose, Lp(a), ApoE and ApoA in males (ALP: 
      r = -0.308, p = 0.042; glucose: r = -0.383, p = 0.010; Lp(a): r = 0.333, 
      p = 0.027; ApoE: r = -0.345, p = 0.032;), ApoA levels in females (r = 0.362, 
      p = 0.033), and C Reactive Protein (CRP) levels in both genders (males: 
      r = -0.373, p = 0.016; females: r = -0.399, p = 0.021). Our work suggested that 
      peripheral BDNF promoter methylation might be a diagnostic marker of AD risk, 
      although its underlying function remains to be elaborated in the future.
FAU - Chang, Lan
AU  - Chang L
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Wang, Yunliang
AU  - Wang Y
AD  - Department of Neurology, the 148 Central Hospital of PLA, Zibo, Shandong, China.
FAU - Ji, Huihui
AU  - Ji H
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Dai, Dongjun
AU  - Dai D
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Xu, Xuting
AU  - Xu X
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Jiang, Danjie
AU  - Jiang D
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Hong, Qingxiao
AU  - Hong Q
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Ye, Huadan
AU  - Ye H
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Zhang, Xiaonan
AU  - Zhang X
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Zhou, Xiaohui
AU  - Zhou X
AD  - Department of Internal Medicine for Cadres, the First Affiliated Hospital of 
      Xinjiang Medical University, Urumchi, China.
FAU - Liu, Yu
AU  - Liu Y
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Li, Jinfeng
AU  - Li J
AD  - Department of Neurology, the 148 Central Hospital of PLA, Zibo, Shandong, China.
FAU - Chen, Zhongming
AU  - Chen Z
AD  - Ningbo Kangning Hospital, Zhejiang, China.
FAU - Li, Ying
AU  - Li Y
AD  - Ningbo No. 1 Hospital, Zhejiang, China.
FAU - Zhou, Dongsheng
AU  - Zhou D
AD  - Ningbo Kangning Hospital, Zhejiang, China.
FAU - Zhuo, Renjie
AU  - Zhuo R
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Zhang, Yuzheng
AU  - Zhang Y
AD  - Department of Neurology, the 148 Central Hospital of PLA, Zibo, Shandong, China.
FAU - Yin, Honglei
AU  - Yin H
AD  - Department of Neurology, the 148 Central Hospital of PLA, Zibo, Shandong, China.
FAU - Mao, Congcong
AU  - Mao C
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Duan, Shiwei
AU  - Duan S
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Wang, Qinwen
AU  - Wang Q
AD  - Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo 
      University, Ningbo, Zhejiang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141103
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Age of Onset
MH  - Aged
MH  - Aged, 80 and over
MH  - Alzheimer Disease/epidemiology/*genetics
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Case-Control Studies
MH  - Comorbidity
MH  - CpG Islands
MH  - *DNA Methylation
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Promoter Regions, Genetic
MH  - Risk
MH  - Risk Factors
PMC - PMC4217733
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/11/05 06:00
MHDA- 2015/08/11 06:00
PMCR- 2014/11/03
CRDT- 2014/11/04 06:00
PHST- 2014/06/12 00:00 [received]
PHST- 2014/09/17 00:00 [accepted]
PHST- 2014/11/04 06:00 [entrez]
PHST- 2014/11/05 06:00 [pubmed]
PHST- 2015/08/11 06:00 [medline]
PHST- 2014/11/03 00:00 [pmc-release]
AID - PONE-D-14-24670 [pii]
AID - 10.1371/journal.pone.0110773 [doi]
PST - epublish
SO  - PLoS One. 2014 Nov 3;9(11):e110773. doi: 10.1371/journal.pone.0110773. 
      eCollection 2014.