PMID- 25365803
OWN - NLM
STAT- MEDLINE
DCOM- 20150724
LR  - 20181113
IS  - 2162-3279 (Electronic)
IS  - 2162-3279 (Print)
VI  - 4
IP  - 6
DP  - 2014
TI  - Type 1 equilibrative nucleoside transporter regulates astrocyte-specific glial 
      fibrillary acidic protein expression in the striatum.
PG  - 903-14
LID - 10.1002/brb3.283 [doi]
AB  - BACKGROUND: Adenosine signaling has been implicated in several neurological and 
      psychiatric disorders. Previously, we found that astrocytic excitatory amino acid 
      transporter 2 (EAAT2) and aquaporin 4 (AQP4) are downregulated in the striatum of 
      mice lacking type 1 equilibrative nucleoside transporter (ENT1). METHODS: To 
      further investigate the gene expression profile in the striatum, we preformed 
      Illumina Mouse Whole Genome BeadChip microarray analysis of the caudate-putamen 
      (CPu) and nucleus accumbens (NAc) in ENT1 null mice. Gene expression was 
      validated by RT-PCR, Western blot, and immunofluorescence. Using transgenic mice 
      expressing enhanced green fluorescence protein (EGFP) under the control of the 
      glial fibrillary acidic protein (GFAP) promoter, we examined EGFP expression in 
      an ENT1 null background. RESULTS: Glial fibrillary acidic protein was identified 
      as a top candidate gene that was reduced in ENT1 null mice compared to wild-type 
      littermates. Furthermore, EGFP expression was significantly reduced in GFAP-EGFP 
      transgenic mice in an ENT1 null background in both the CPu and NAc. Finally, 
      pharmacological inhibition or siRNA knockdown of ENT1 in cultured astrocytes also 
      reduced GFAP mRNA levels. CONCLUSIONS: Overall, our findings demonstrate that 
      ENT1 regulates GFAP expression and possibly astrocyte function.
FAU - Hinton, David J
AU  - Hinton DJ
AD  - Department of Psychiatry and Psychology, Mayo Clinic, College of Medicine 
      Rochester, Minnesota, 55905 ; Department of Molecular Pharmacology and 
      Experimental Therapeutics, Mayo Clinic, College of Medicine Rochester, Minnesota, 
      55905 ; Neurobiology of Disease Program, Mayo Clinic, College of Medicine 
      Rochester, Minnesota, 55905.
FAU - Lee, Moonnoh R
AU  - Lee MR
AD  - Department of Biochemistry and Molecular Biology, Mayo Clinic, College of 
      Medicine Rochester, Minnesota, 55905.
FAU - Jang, Jin Sung
AU  - Jang JS
AD  - Division of Pulmonary and Critical Care Medicine, Mayo Clinic, College of 
      Medicine Rochester, Minnesota, 55905.
FAU - Choi, Doo-Sup
AU  - Choi DS
AD  - Department of Psychiatry and Psychology, Mayo Clinic, College of Medicine 
      Rochester, Minnesota, 55905 ; Department of Molecular Pharmacology and 
      Experimental Therapeutics, Mayo Clinic, College of Medicine Rochester, Minnesota, 
      55905 ; Neurobiology of Disease Program, Mayo Clinic, College of Medicine 
      Rochester, Minnesota, 55905.
LA  - eng
GR  - R01 AA018779/AA/NIAAA NIH HHS/United States
GR  - UL1 TR000135/TR/NCATS NIH HHS/United States
GR  - AA018779/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140919
PL  - United States
TA  - Brain Behav
JT  - Brain and behavior
JID - 101570837
RN  - 0 (Equilibrative Nucleoside Transporter 1)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SLC29A1 protein, mouse)
RN  - 0 (enhanced green fluorescent protein)
RN  - 0 (glial fibrillary astrocytic protein, mouse)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Blotting, Western
MH  - Cell Line
MH  - Corpus Striatum/*metabolism
MH  - Equilibrative Nucleoside Transporter 1/antagonists & 
      inhibitors/genetics/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Profiling
MH  - Glial Fibrillary Acidic Protein
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Male
MH  - Mice, 129 Strain
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Microarray Analysis
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC4178301
OTO - NOTNLM
OT  - Adenosine transporter
OT  - ENT1
OT  - GFAP
OT  - astrocyte
OT  - microarray
EDAT- 2014/11/05 06:00
MHDA- 2015/07/25 06:00
PMCR- 2014/11/01
CRDT- 2014/11/04 06:00
PHST- 2014/02/05 00:00 [received]
PHST- 2014/06/30 00:00 [revised]
PHST- 2014/08/26 00:00 [accepted]
PHST- 2014/11/04 06:00 [entrez]
PHST- 2014/11/05 06:00 [pubmed]
PHST- 2015/07/25 06:00 [medline]
PHST- 2014/11/01 00:00 [pmc-release]
AID - BRB3283 [pii]
AID - 10.1002/brb3.283 [doi]
PST - ppublish
SO  - Brain Behav. 2014;4(6):903-14. doi: 10.1002/brb3.283. Epub 2014 Sep 19.