PMID- 25367204
OWN - NLM
STAT- MEDLINE
DCOM- 20151130
LR  - 20211021
IS  - 1573-9368 (Electronic)
IS  - 0962-8819 (Linking)
VI  - 24
IP  - 2
DP  - 2015 Apr
TI  - Effects of transgenic sterilization constructs and their repressor compounds on 
      hatch, developmental rate and early survival of electroporated channel catfish 
      embryos and fry.
PG  - 333-52
LID - 10.1007/s11248-014-9846-4 [doi]
AB  - Channel catfish (Ictalurus punctatus) embryos were electroporated with 
      sterilization constructs targeting primordial germ cell proteins or with buffer. 
      Some embryos then were treated with repressor compounds, cadmium chloride, copper 
      sulfate, sodium chloride or doxycycline, to prevent expression of the transgene 
      constructs. Promoters included channel catfish nanos and vasa, salmon transferrin 
      (TF), modified yeast Saccharomyces cerevisiae copper transport protein (MCTR) and 
      zebrafish racemase (RM). Knock-down systems were the Tet-off (nanos and vasa 
      constructs), MCTR, RM and TF systems. Knock-down genes included shRNAi targeting 
      5' nanos (N1), 3' nanos (N2) or dead end (DND), or double-stranded nanos RNA 
      (dsRNA) for overexpression of nanos mRNA. These constructs previously were 
      demonstrated to knock down nanos, vasa and dead end, with the repressors having 
      variable success. Exogenous DNA affected percentage hatch (% hatch), as all 14 
      constructs, except for the TF dsRNA, TF N1 (T), RM DND (C), vasa DND (C), vasa N1 
      (C) and vasa N2 (C), had lower % hatch than the control electroporated with 
      buffer. The MCTR and RM DND (T) constructs resulted in delayed hatch, and the 
      vasa and nanos constructs had minimal effects on time of hatch (P < 0.05). 
      Cadmium chloride appeared to counteract the slow development caused by the TF 
      constructs in two TF treatments (P < 0.05). The 4 ppt sodium chloride treatment 
      for the RM system decreased % hatch (P < 0.05) and slowed development. In the 
      case of nanos constructs, doxycycline greatly delayed hatch (P < 0.05). Adverse 
      effects of the transgenes and repressors continued for several treatments for the 
      first 6 days after hatch, but only in a few treatments during the next 10 days. 
      Repressors and gene expression impacted the yield of putative transgenic channel 
      catfish fry, and need to be considered and accounted for in the hatchery phase of 
      producing transgenically sterilized catfish fry and their fertile counterparts. 
      This fry output should be considered to ensure that sufficient numbers of 
      transgenic fish are produced for future applications and for defining repressor 
      systems that are the most successful.
FAU - Su, Baofeng
AU  - Su B
AD  - School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 
      Auburn, AL, 36849, USA, subfeng@auburn.edu.
FAU - Shang, Mei
AU  - Shang M
FAU - Li, Chao
AU  - Li C
FAU - Perera, Dayan A
AU  - Perera DA
FAU - Pinkert, Carl A
AU  - Pinkert CA
FAU - Irwin, Michael H
AU  - Irwin MH
FAU - Peatman, Eric
AU  - Peatman E
FAU - Grewe, Peter
AU  - Grewe P
FAU - Patil, Jawahar G
AU  - Patil JG
FAU - Dunham, Rex A
AU  - Dunham RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20141104
PL  - Netherlands
TA  - Transgenic Res
JT  - Transgenic research
JID - 9209120
RN  - 0 (RNA, Messenger)
RN  - EC 5.1.- (Racemases and Epimerases)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Catfishes/*genetics/growth & development
MH  - Embryo, Nonmammalian
MH  - Gene Expression Regulation, Developmental
MH  - Germ Cells/drug effects/*growth & development
MH  - RNA, Messenger/biosynthesis
MH  - Racemases and Epimerases/administration & dosage
MH  - Reproduction/*genetics
MH  - *Transgenes
MH  - Zebrafish/genetics/metabolism
EDAT- 2014/11/05 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/11/05 06:00
PHST- 2014/06/03 00:00 [received]
PHST- 2014/10/27 00:00 [accepted]
PHST- 2014/11/05 06:00 [entrez]
PHST- 2014/11/05 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1007/s11248-014-9846-4 [doi]
PST - ppublish
SO  - Transgenic Res. 2015 Apr;24(2):333-52. doi: 10.1007/s11248-014-9846-4. Epub 2014 
      Nov 4.