PMID- 25368812
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141104
LR  - 20211021
IS  - 2093-2278 (Print)
IS  - 2093-2286 (Electronic)
IS  - 2093-2278 (Linking)
VI  - 44
IP  - 5
DP  - 2014 Oct
TI  - Human CD103(+) dendritic cells promote the differentiation of Porphyromonas 
      gingivalis heat shock protein peptide-specific regulatory T cells.
PG  - 235-41
LID - 10.5051/jpis.2014.44.5.235 [doi]
AB  - PURPOSE: Regulatory T cells (Tregs), expressing CD4 and CD25 as well as Foxp3, 
      are known to play a pivotal role in immunoregulatory function in autoimmune 
      diseases, cancers, and graft rejection. Dendritic cells (DCs) are considered the 
      major antigen-presenting cells (APCs) for initiating these T-cell immune 
      responses, of which CD103(+) DCs are derived from precursor human peripheral 
      blood mononuclear cells (PBMCs). The aim of the present study was to evaluate the 
      capacity of these PBMC-derived CD103(+) DCs to promote the differentiation of 
      antigen-specific Tregs. METHODS: Monocyte-derived DCs were induced from CD14(+) 
      monocytes from the PBMCs of 10 healthy subjects. Once the CD103(+) DCs were 
      purified, the cell population was enriched by adding retinoic acid (RA). Peptide 
      numbers 14 and 19 of Porphyromonas gingivalis heat shock protein 60 (HSP60) were 
      synthesized to pulse CD103(+) DCs as a tool for presenting the peptide antigens 
      to stimulate CD3(+) T cells that were isolated from human PBMC. Exogenous 
      interleukin 2 was added as a coculture supplement. The antigen-specific T-cell 
      lines established were phenotypically identified for their expression of CD4, 
      CD25, or Foxp3. RESULTS: When PBMCs were used as APCs, they demonstrated only a 
      marginal capacity to stimulate peptide-specific Tregs, whereas CD103(+) DCs 
      showed a potent antigen presenting capability to promote the peptide-specific 
      Tregs, especially for peptide 14. RA enhanced the conversion of CD103(+) DCs, 
      which paralleled the antigen-specific Treg-stimulating effect, though the 
      differences failed to reach statistical significance. CONCLUSIONS: We 
      demonstrated that CD103(+) DCs can promote antigen-specific Tregs from naive T 
      cells, when used as APCs for an epitope peptide from P. gingivalis HSP60. RA was 
      an effective reagent that induces mature DCs with the typical phenotypic 
      expression of CD103 that demonstrated the functional capability to promote 
      antigen-specific Tregs.
FAU - Kim, Myung-Jin
AU  - Kim MJ
AUID- ORCID: 0000-0003-0528-6848
AD  - Department of Periodontology, Pusan University School of Dentistry, Yangsan, 
      Korea.
FAU - Jeong, Eui-Kyong
AU  - Jeong EK
AUID- ORCID: 0000-0001-9866-9842
AD  - Department of Molecular Biology, Pusan University College of Natural Sciences, 
      Yangsan, Korea.
FAU - Kwon, Eun-Young
AU  - Kwon EY
AUID- ORCID: 0000-0001-9555-0360
AD  - Department of Periodontology, Pusan University School of Dentistry, Yangsan, 
      Korea.
FAU - Joo, Ji-Young
AU  - Joo JY
AUID- ORCID: 0000-0002-4050-5797
AD  - Department of Periodontology, Pusan University School of Dentistry, Yangsan, 
      Korea.
FAU - Lee, Ju-Youn
AU  - Lee JY
AUID- ORCID: 0000-0002-0772-033X
AD  - Department of Periodontology, Pusan University School of Dentistry, Yangsan, 
      Korea.
FAU - Choi, Jeomil
AU  - Choi J
AUID- ORCID: 0000-0002-7491-6711
AD  - Department of Periodontology, Pusan University School of Dentistry, Yangsan, 
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20141029
PL  - Korea (South)
TA  - J Periodontal Implant Sci
JT  - Journal of periodontal & implant science
JID - 101526931
PMC - PMC4216400
OTO - NOTNLM
OT  - Autoimmune diseases
OT  - Cells
OT  - Immunity
OT  - Periodontitis
OT  - Proteins
COIS- No potential conflict of interest relevant to this article was reported.
EDAT- 2014/11/05 06:00
MHDA- 2014/11/05 06:01
PMCR- 2014/10/01
CRDT- 2014/11/05 06:00
PHST- 2014/07/26 00:00 [received]
PHST- 2014/09/24 00:00 [accepted]
PHST- 2014/11/05 06:00 [entrez]
PHST- 2014/11/05 06:00 [pubmed]
PHST- 2014/11/05 06:01 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - 10.5051/jpis.2014.44.5.235 [doi]
PST - ppublish
SO  - J Periodontal Implant Sci. 2014 Oct;44(5):235-41. doi: 
      10.5051/jpis.2014.44.5.235. Epub 2014 Oct 29.