PMID- 2536931
OWN - NLM
STAT- MEDLINE
DCOM- 19890316
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 86
IP  - 3
DP  - 1989 Feb
TI  - Platelet-derived growth factor synthesis in mesangial cells: induction by 
      multiple peptide mitogens.
PG  - 1056-60
AB  - Platelet-derived growth factor (PDGF) has been implicated in several nonmalignant 
      pathophysiological processes, including proliferative diseases of the kidney. 
      Glomerular mesangial cells secrete a PDGF-like factor and express the PDGF 
      A-chain and c-sis (or B-chain) mRNAs. We report here that both mRNAs are induced 
      by serum and this effect can be mimicked by recombinant PDGF, which also markedly 
      stimulates DNA synthesis. Other growth factors, such as epidermal growth factor 
      (EGF), transforming growth factor type alpha, basic fibroblast growth factor 
      (bFGF), and tumor necrosis factor type alpha (TNF-alpha) also are mitogenic for 
      human mesangial cells and induce expression of the PDGF mRNAs. EGF, TNF-alpha, 
      and bFGF also stimulate these cells to secrete a PDGF-like factor. Furthermore, 
      anti-PDGF antibody partially abrogates the mitogenic effect of EGF, suggesting 
      that mitogen-stimulated PDGF synthesis in mesangial cells is at least partly 
      responsible for cell growth induced by other growth factors. In contrast to these 
      results, transforming growth factor type beta (TGF-beta), while inducing both 
      mRNAs, is not mitogenic, indicating that its effect on message levels can be 
      dissociated from DNA synthesis. These data suggest that several peptide growth 
      factors regulate the growth of mesangial cells and that PDGF may be an effector 
      molecule that plays a role in the mitogenic response to many of these growth 
      stimuli.
FAU - Silver, B J
AU  - Silver BJ
AD  - Division of Hematology/Oncology, Cleveland Veterans Administration, Case Western 
      Reserve University, OH 44106.
FAU - Jaffer, F E
AU  - Jaffer FE
FAU - Abboud, H E
AU  - Abboud HE
LA  - eng
GR  - AM07470/AM/NIADDK NIH HHS/United States
GR  - DK33665/DK/NIDDK NIH HHS/United States
GR  - P30CA43703/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Macromolecular Substances)
RN  - 0 (Mitogens)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - EC 2.7.10.1 (Receptors, Platelet-Derived Growth Factor)
SB  - IM
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - DNA Replication
MH  - Gene Expression Regulation/drug effects
MH  - Genes
MH  - Glomerular Mesangium/drug effects/*metabolism
MH  - Humans
MH  - Macromolecular Substances
MH  - Mitogens/*pharmacology
MH  - Platelet-Derived Growth Factor/biosynthesis/*genetics
MH  - RNA, Messenger/drug effects/genetics
MH  - Receptors, Cell Surface/metabolism
MH  - Receptors, Platelet-Derived Growth Factor
PMC - PMC286620
EDAT- 1989/02/01 00:00
MHDA- 1989/02/01 00:01
PMCR- 1989/08/01
CRDT- 1989/02/01 00:00
PHST- 1989/02/01 00:00 [pubmed]
PHST- 1989/02/01 00:01 [medline]
PHST- 1989/02/01 00:00 [entrez]
PHST- 1989/08/01 00:00 [pmc-release]
AID - 10.1073/pnas.86.3.1056 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1989 Feb;86(3):1056-60. doi: 10.1073/pnas.86.3.1056.