PMID- 25371071
OWN - NLM
STAT- MEDLINE
DCOM- 20150615
LR  - 20221207
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 36
IP  - 3
DP  - 2015 Mar
TI  - Matrine inhibits the growth and induces apoptosis of osteosarcoma cells in vitro 
      by inactivating the Akt pathway.
PG  - 1653-9
LID - 10.1007/s13277-014-2764-5 [doi]
AB  - Matrine, a natural product, has been demonstrated to be a promising 
      chemotherapeutic drug for some cancers. Using flow cytometric analysis of the 
      cell cycle and apoptosis, we found that matrine inhibited the proliferation and 
      induced apoptosis in the human osteosarcoma (OS) cell lines MG63, HOS, U2OS, and 
      SAOS2 in vitro in a dose-dependent manner. We therefore assessed the role of the 
      serine/threonine kinase Akt in the regulation of matrine-mediated cell growth 
      inhibition and apoptosis induction in human OS cell lines. After treatment for 48 
      h, matrine induced G0/G1-stage cell cycle arrest in MG63, U2OS, and SAOS2 cells 
      associated with an increase in the expression of p27(Kip1) and a decrease in the 
      expression of Akt, glycogen synthase kinase 3 (GSK3)-beta (Ser9), and cyclin D1. 
      Furthermore, the pro-apoptotic factor Bax was upregulated. Overall, our findings 
      suggest that matrine may be an effective anti-osteosarcoma drug due to its 
      ability to inhibit proliferation and induce apoptosis in OS cells, possibly 
      through the involvement of Akt signaling.
FAU - Xu, Gong-Ping
AU  - Xu GP
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, 148 Baojian Road, Harbin, 150081, China.
FAU - Zhao, Wei
AU  - Zhao W
FAU - Zhuang, Jin-Peng
AU  - Zhuang JP
FAU - Zu, Jia-Ning
AU  - Zu JN
FAU - Wang, Duan-Yang
AU  - Wang DY
FAU - Han, Fei
AU  - Han F
FAU - Zhang, Zhi-Peng
AU  - Zhang ZP
FAU - Yan, Jing-Long
AU  - Yan JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141105
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Alkaloids)
RN  - 0 (BAX protein, human)
RN  - 0 (CDKN1B protein, human)
RN  - 0 (Quinolizines)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 136601-57-5 (Cyclin D1)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
RN  - 0 (Matrines)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Bone Neoplasms/*drug therapy/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cyclin D1/metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p27/metabolism
MH  - G1 Phase Cell Cycle Checkpoints/drug effects
MH  - Glycogen Synthase Kinase 3/metabolism
MH  - Glycogen Synthase Kinase 3 beta
MH  - Humans
MH  - Osteosarcoma/*drug therapy/metabolism/pathology
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Quinolizines/*pharmacology
MH  - Resting Phase, Cell Cycle/drug effects
MH  - Signal Transduction/*drug effects
MH  - bcl-2-Associated X Protein/metabolism
MH  - Matrines
EDAT- 2014/11/06 06:00
MHDA- 2015/06/16 06:00
CRDT- 2014/11/06 06:00
PHST- 2014/08/10 00:00 [received]
PHST- 2014/10/22 00:00 [accepted]
PHST- 2014/11/06 06:00 [entrez]
PHST- 2014/11/06 06:00 [pubmed]
PHST- 2015/06/16 06:00 [medline]
AID - 10.1007/s13277-014-2764-5 [doi]
PST - ppublish
SO  - Tumour Biol. 2015 Mar;36(3):1653-9. doi: 10.1007/s13277-014-2764-5. Epub 2014 Nov 
      5.