PMID- 25371750 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20211021 IS - 1792-0981 (Print) IS - 1792-1015 (Electronic) IS - 1792-0981 (Linking) VI - 8 IP - 6 DP - 2014 Dec TI - Brain-derived neurotrophic factor exerts neuroprotective actions against amyloid beta-induced apoptosis in neuroblastoma cells. PG - 1891-1895 AB - Alzheimer's disease (AD) brains demonstrate decreased levels of brain-derived neurotrophic factor (BDNF) and increased levels of beta-amyloid peptide (Abeta), which is neurotoxic. The present study assessed the impact of BDNF on the toxic effects of Abeta(25-35)-induced apoptosis and the effects on BDNF-mediated signaling using the MTT assay, western blotting and reverse transcription quantitative polymerase chain reaction. Abeta(25-35) was found to induce an apoptosis, dose-dependent effect on SH-SY5Y neuroblastoma cells, which peaked at a concentration of 20 muM after 24 h. A combination of Abeta(25-35) and BDNF treatment increased the levels of Akt and decreased the level of glycogen synthase kinase-3beta (GSK3beta) in SH-SY5Y neuroblastoma cells. These findings indicated that BDNF administration exerted a neuroprotective effect against the toxicity of the Abeta(25-35)-induced apoptosis in these cells, which was accompanied by phosphoinositide 3-kinase/Akt activation and GSK3beta phosphorylation. The mechanisms and signaling pathways underlying neuronal degeneration induced by the Abeta peptide remain to be further elucidated. FAU - Kim, Jin Hee AU - Kim JH AD - Department of Neurosurgery, College of Medicine, Korea University, Seoul 136-701, Republic of Korea. LA - eng PT - Journal Article DEP - 20141017 PL - Greece TA - Exp Ther Med JT - Experimental and therapeutic medicine JID - 101531947 PMC - PMC4218707 OTO - NOTNLM OT - Akt OT - brain-derived neurotrophic factor OT - glycogen synthase kinase 3beta OT - neuroblastoma cell OT - beta-amyloid peptide EDAT- 2014/11/06 06:00 MHDA- 2014/11/06 06:01 PMCR- 2014/10/17 CRDT- 2014/11/06 06:00 PHST- 2014/02/20 00:00 [received] PHST- 2014/08/13 00:00 [accepted] PHST- 2014/11/06 06:00 [entrez] PHST- 2014/11/06 06:00 [pubmed] PHST- 2014/11/06 06:01 [medline] PHST- 2014/10/17 00:00 [pmc-release] AID - etm-08-06-1891 [pii] AID - 10.3892/etm.2014.2033 [doi] PST - ppublish SO - Exp Ther Med. 2014 Dec;8(6):1891-1895. doi: 10.3892/etm.2014.2033. Epub 2014 Oct 17.