PMID- 25372971
OWN - NLM
STAT- MEDLINE
DCOM- 20150629
LR  - 20181113
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 20
DP  - 2014 Nov 5
TI  - Oxygen tension variation in ischemic gastrocnemius muscle, marrow, and different 
      hypoxic conditions in vitro.
PG  - 2171-6
LID - 10.12659/MSM.892354 [doi]
AB  - BACKGROUND: Bone marrow stromal cells (BMSCs) play an important role in ischemic 
      limb angiogenesis. BMSCs cultured in vitro can be exposed to oxygen tension much 
      higher than that experienced in vivo. This study assessed oxygen tension in bone 
      marrow and ischemic muscle in vivo, and then identified an appropriate oxygen 
      concentration for culturing BMSCs. MATERIAL/METHODS: Unilateral hind limb 
      ischemia was surgically induced in 30 mice, and tissue oxygen tension in 
      bilateral gastrocnemius muscles and femoral bone marrow was monitored in vivo 
      using a micro-electrode at 24 hours, 1 week, 2 weeks, and 3 weeks after modeling. 
      Media used for culturing normal marrow, muscle, and artery tissue were incubated 
      with various oxygen concentrations, and O2 tension was continuously monitored. 
      Oxygen tension in aortic arterial blood was monitored using a micro-electrode and 
      blood gas analyzer, and the results were compared. RESULTS: Oxygen tension in 
      ischemic gastrocnemius muscle reached a nadir at 1 week after ischemic modeling, 
      when histological changes were most noticeable. Culture media incubated with 3%, 
      6%, and 14% oxygen (the normal oxygen levels of bone marrow, muscle, and arterial 
      blood, respectively) required 9, 6, and 2 hours, respectively, to reach an 
      equilibrated oxygen tension, and oxygen tension was elevated by 1.6-, 1.2-, and 
      0.4-fold, respectively, upon re-exposure of the media to air. CONCLUSIONS: 
      Physiological oxygen tension differs in different tissues. A 3% O2 concentration 
      mimics the physiological O2 exposure experienced by BMSCs and represents the 
      hypoxic concentration. Culture medium incubated under hypoxic conditions requires 
      a prolonged period of time to regain equilibrated oxygen tension.
FAU - Huang, Ying
AU  - Huang Y
AD  - Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, 
      Beijing, China (mainland).
FAU - Chen, Bing
AU  - Chen B
AD  - Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, 
      Beijing, China (mainland).
FAU - Zhang, Jian
AU  - Zhang J
AD  - Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, 
      Beijing, China (mainland).
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141105
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Culture Media)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Arteries/metabolism
MH  - Bone Marrow/*blood supply/pathology
MH  - Culture Media/chemistry
MH  - Femur/drug effects/pathology
MH  - Hypoxia/*complications/*metabolism
MH  - Ischemia/*complications/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Muscle, Skeletal/*blood supply/pathology
MH  - Oxygen/*metabolism
MH  - Time Factors
PMC - PMC4301230
EDAT- 2014/11/06 06:00
MHDA- 2015/06/30 06:00
PMCR- 2014/11/05
CRDT- 2014/11/06 06:00
PHST- 2014/11/06 06:00 [entrez]
PHST- 2014/11/06 06:00 [pubmed]
PHST- 2015/06/30 06:00 [medline]
PHST- 2014/11/05 00:00 [pmc-release]
AID - 892354 [pii]
AID - 10.12659/MSM.892354 [doi]
PST - epublish
SO  - Med Sci Monit. 2014 Nov 5;20:2171-6. doi: 10.12659/MSM.892354.