PMID- 25374393
OWN - NLM
STAT- MEDLINE
DCOM- 20150921
LR  - 20220331
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 32
IP  - 1
DP  - 2015 Jan
TI  - DNA fragmentation in human sperm after magnetic-activated cell sorting.
PG  - 147-54
LID - 10.1007/s10815-014-0370-5 [doi]
AB  - PURPOSE: As fertilization with unselected apoptotic spermatozoa may contribute to 
      failures in assisted reproductive techniques, it has become essential to remove 
      this type of sperm in order to increase the success rates. Magnetic-activated 
      cell sorting (MACS) is a sperm preparation technique that isolates non-apoptotic 
      spermatozoa based on the expression of phosphatidylserine in the membrane of 
      apoptotic sperm. Therefore, we aimed to evaluate whether there was a significant 
      decrease in sperm DNA fragmentation (sDNAfrag) and verify which protocol was the 
      most efficient. METHODS: Hundred semen samples were allocated into five distinct 
      groups and processed according to a combination of MACS with density gradient 
      centrifugation (DGC) and swim-up (SU) techniques. Sperm DNA fragmentation was 
      evaluated by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) 
      assay. RESULTS: Groups DGC-SU (73.4 %), DGC-MACS-SU (78.9 %), DGC-SU-MACS (53.8 
      %) and MACS-SU (73.5 %) presented a significant decrease in sDNAfrag but the 
      highest reduction rate was obtained with MACS-DGC-SU (83.3 %). The later was also 
      negatively correlated with sperm vitality, membrane integrity and progressive 
      motility. Additionally, teratozoospermic patients presented a tendency to have 
      lower sDNAfrag reduction rates than asthenozoospermic and asthenoteratozoospermic 
      patients. CONCLUSIONS: Based on the results, MACS showed potential to optimize 
      the sDNAfrag reduction rate, when applied to raw semen, before DGC and SU, 
      especially in samples with low values of progressive motility, vitality and 
      hypoosmotic swelling test.
FAU - Bucar, Sara
AU  - Bucar S
AD  - Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical 
      Sciences Abel Salazar (ICBAS), University of Porto (UP), Multidisciplinary Unit 
      for Biomedical Research (UMIB), Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, 
      Portugal.
FAU - Goncalves, Ana
AU  - Goncalves A
FAU - Rocha, Eduardo
AU  - Rocha E
FAU - Barros, Alberto
AU  - Barros A
FAU - Sousa, Mario
AU  - Sousa M
FAU - Sa, Rosalia
AU  - Sa R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141106
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Adult
MH  - Asthenozoospermia/*genetics/pathology
MH  - *Cell Separation
MH  - *DNA Fragmentation
MH  - Humans
MH  - Magnetics
MH  - Male
MH  - Sperm Motility/physiology
MH  - *Spermatozoa
PMC - PMC4294881
EDAT- 2014/11/07 06:00
MHDA- 2015/09/22 06:00
PMCR- 2016/01/01
CRDT- 2014/11/07 06:00
PHST- 2014/07/17 00:00 [received]
PHST- 2014/10/13 00:00 [accepted]
PHST- 2014/11/07 06:00 [entrez]
PHST- 2014/11/07 06:00 [pubmed]
PHST- 2015/09/22 06:00 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 370 [pii]
AID - 10.1007/s10815-014-0370-5 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2015 Jan;32(1):147-54. doi: 10.1007/s10815-014-0370-5. 
      Epub 2014 Nov 6.