PMID- 25377434
OWN - NLM
STAT- MEDLINE
DCOM- 20150511
LR  - 20220310
IS  - 1435-702X (Electronic)
IS  - 0721-832X (Linking)
VI  - 252
IP  - 12
DP  - 2014 Dec
TI  - Hyalocytes in idiopathic epiretinal membranes: a correlative light and electron 
      microscopic study.
PG  - 1887-94
LID - 10.1007/s00417-014-2841-x [doi]
AB  - PURPOSE: To describe characteristics of epiretinal cells at the vitreoretinal 
      interface by correlative light and electron microscopy (CLEM). METHODS: 
      Epiretinal membrane (ERM) specimens and internal limiting membrane (ILM) 
      specimens were harvested by sequential peeling during vitrectomy from 27 eyes 
      with idiopathic epiretinal gliosis, and processed for CLEM. Intraoperatively, the 
      presence of posterior vitreous detachment (PVD) was documented. We used 
      anti-vimentin, anti-alpha-smooth muscle actin (alpha-SMA), and anti-CD45 as primary 
      antibodies. A fluorescein-tagged immunonanogold cluster was used as secondary 
      antibody and visualized under the fluorescence and transmission electron 
      microscope. RESULTS: We demonstrated CD45-positive cells specifically labelled at 
      their plasma membranes with ultrastructural features known for hyalocytes, such 
      as oval nucleus with marginal chromatin, vacuoles, dense granules, and thin 
      cytoplasmic protrusions. CD45-positive cells were mostly located on a thick layer 
      of native vitreous collagen. They were covered by newly formed collagen strands 
      with multilayered proliferation of myofibroblasts. We also demonstrated 
      immunoreactivity for vimentin and alpha-SMA. Cell fragments with positive 
      labelling for alpha-SMA and vimentin were not only found on the vitreal side of the 
      ILM, but also on the retinal side. CONCLUSIONS: By CLEM, the majority of 
      CD45-positive cells in epiretinal cell proliferation were characterized as 
      hyalocytes. In the context of anomalous PVD and vitreoschisis, ultrastructural 
      features and topographic localization of hyalocytes suggest that these cells play 
      a significant role in ERM formation. CLEM enables a more accurate 
      characterization of epiretinal cell proliferation, and therefore, contributes to 
      a better understanding of the pathogenesis of diseases at the vitreoretinal 
      interface.
FAU - Schumann, Ricarda G
AU  - Schumann RG
AD  - Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany, 
      ricarda.schumann@med.uni-muenchen.de.
FAU - Gandorfer, Arnd
AU  - Gandorfer A
FAU - Ziada, Jean
AU  - Ziada J
FAU - Scheler, Renate
AU  - Scheler R
FAU - Schaumberger, Markus M
AU  - Schaumberger MM
FAU - Wolf, Armin
AU  - Wolf A
FAU - Kampik, Anselm
AU  - Kampik A
FAU - Haritoglou, Christos
AU  - Haritoglou C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141107
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
RN  - 0 (ACTA2 protein, human)
RN  - 0 (Actins)
RN  - 0 (Biomarkers)
RN  - 0 (Vimentin)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.1.3.48 (PTPRC protein, human)
SB  - IM
MH  - Actins/metabolism
MH  - Aged
MH  - Aged, 80 and over
MH  - Basement Membrane/*ultrastructure
MH  - Biomarkers/metabolism
MH  - Cell Proliferation
MH  - Epiretinal Membrane/metabolism/*pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Leukocyte Common Antigens/metabolism
MH  - Male
MH  - Microscopy, Electron
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - Vimentin/metabolism
MH  - Vitrectomy
MH  - Vitreous Body/*cytology
MH  - Vitreous Detachment/diagnosis
EDAT- 2014/11/08 06:00
MHDA- 2015/05/12 06:00
CRDT- 2014/11/08 06:00
PHST- 2014/08/01 00:00 [received]
PHST- 2014/10/21 00:00 [accepted]
PHST- 2014/10/07 00:00 [revised]
PHST- 2014/11/08 06:00 [entrez]
PHST- 2014/11/08 06:00 [pubmed]
PHST- 2015/05/12 06:00 [medline]
AID - 10.1007/s00417-014-2841-x [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 2014 Dec;252(12):1887-94. doi: 
      10.1007/s00417-014-2841-x. Epub 2014 Nov 7.