PMID- 25377868
OWN - NLM
STAT- MEDLINE
DCOM- 20150710
LR  - 20181202
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 48
IP  - 24
DP  - 2014 Dec 16
TI  - Dehalococcoides mccartyi strain JNA dechlorinates multiple chlorinated phenols 
      including pentachlorophenol and harbors at least 19 reductive dehalogenase 
      homologous genes.
PG  - 14300-8
LID - 10.1021/es503553f [doi]
AB  - Pentachlorophenol and other chlorinated phenols are highly toxic ubiquitous 
      environmental pollutants. Using gas chromatographic analysis we determined that 
      Dehalococcoides mccartyi strain JNA in pure culture dechlorinated 
      pentachlorophenol to 3,5-dichlorophenol (DCP) via removal of the ortho and para 
      chlorines in all of the three possible pathways. In addition, JNA dechlorinated 
      2,3,4,6-tetrachlorophenol via 2,4,6-trichlorophenol (TCP) and 2,4,5-TCP to 
      2,4-DCP and 3,4-DCP, respectively, and dechlorinated 2,3,6-TCP to 3-chlorophenol 
      (CP) via 2,5-DCP. JNA converted 2,3,4-TCP to 3,4-DCP and 2,4-DCP by ortho and 
      meta dechlorination, respectively. 2,3-DCP was dechlorinated to 3-CP, and, 
      because cultures using it could be transferred with a low inoculum (0.5 to 1.5% 
      vol/vol), it may act as an electron acceptor to support growth. Using PCR 
      amplification with targeted and degenerate primers followed by cloning and 
      sequencing, we determined that JNA harbors at least 19 reductive dehalogenase 
      homologous (rdh) genes including orthologs of pcbA4 and pcbA5, pceA, and mbrA, 
      but not tceA or vcrA. Many of these genes are shared with D. mccartyi strains 
      CBDB1, DCMB5, GT, and CG5. Strain JNA has previously been shown to extensively 
      dechlorinate the commercial polychlorinated biphenyl (PCB) mixture Aroclor 1260. 
      Collectively the data suggest that strain JNA may be well adapted to survive in 
      sites contaminated with chlorinated aromatics and may be useful for in situ 
      bioremediation.
FAU - Fricker, Ashwana D
AU  - Fricker AD
AD  - Department of Biological Sciences and double daggerDepartment of Civil and Environmental 
      Engineering, Rensselaer Polytechnic Institute , 110 8th Street, Troy, New York 
      12180, United States.
FAU - LaRoe, Sarah L
AU  - LaRoe SL
FAU - Shea, Michael E
AU  - Shea ME
FAU - Bedard, Donna L
AU  - Bedard DL
LA  - eng
PT  - Journal Article
DEP - 20141124
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Chlorophenols)
RN  - 0 (DNA Primers)
RN  - 0 (Environmental Pollutants)
SB  - IM
MH  - Bacteria/*enzymology/*genetics/metabolism
MH  - Biodegradation, Environmental
MH  - Chlorophenols/chemistry/*metabolism
MH  - DNA Primers/metabolism
MH  - Environmental Pollutants/metabolism
MH  - Gene Expression Regulation, Bacterial/physiology
MH  - Molecular Structure
MH  - Polymerase Chain Reaction/methods
EDAT- 2014/11/08 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/11/08 06:00
PHST- 2014/11/08 06:00 [entrez]
PHST- 2014/11/08 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1021/es503553f [doi]
PST - ppublish
SO  - Environ Sci Technol. 2014 Dec 16;48(24):14300-8. doi: 10.1021/es503553f. Epub 
      2014 Nov 24.