PMID- 25380357 OWN - NLM STAT- MEDLINE DCOM- 20150921 LR - 20150107 IS - 1943-3700 (Electronic) IS - 0090-3558 (Linking) VI - 51 IP - 1 DP - 2015 Jan TI - Multiplex serology for common viral infections in feral pigs (Sus scrofa) in Hawaii between 2007 and 2010. PG - 239-43 LID - 10.7589/2013-09-242 [doi] AB - Multiplex serology was performed for the detection of total immunoglobulin (Ig) and IgM antibodies against porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and swine influenza virus (SIV) antigens in feral swine (Sus scrofa). Serum samples were collected from the islands of Oahu (292 pigs) and Hawaii (52 pigs) between 2007 and 2010. The highest antibody prevalence was to PCV2 (63%), followed by SIV (7.8%) and PRRSV (5.8%). Antigen-specific IgM was detected at a much lower prevalence. PCR amplification and sequence analysis of PCV2 in three IgM-positive samples identified PCV2b as the only genotype. While the prevalence of PCV2 and PRRSV remained similar between 2007 and 2010, the percentage of SIV-positive samples on Oahu increased from 2% to 19%. Our results demonstrate the utility of multiplex serology for pathogen surveillance in feral pig populations. FAU - Stephenson, Rachel J AU - Stephenson RJ AD - 1 School of Chemistry and Molecular Biosciences, University of Queensland, Chemistry Building 68 Cooper Rd., Brisbane, QLD 4072, Australia. FAU - Trible, Benjamin R AU - Trible BR FAU - Wang, Yu AU - Wang Y FAU - Kerrigan, Maureen A AU - Kerrigan MA FAU - Goldstein, Samuel M AU - Goldstein SM FAU - Rowland, Raymond R R AU - Rowland RR LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Wildl Dis JT - Journal of wildlife diseases JID - 0244160 RN - 0 (Antibodies, Viral) RN - 0 (Immunoglobulin M) SB - IM MH - Animals MH - Animals, Wild MH - Antibodies, Viral/blood MH - Circovirus/genetics/immunology MH - Hawaii/epidemiology MH - Immunoglobulin M/blood MH - Influenza A virus/immunology MH - Multiplex Polymerase Chain Reaction/methods/*veterinary MH - Porcine respiratory and reproductive syndrome virus/immunology MH - Serologic Tests/veterinary MH - Swine MH - Swine Diseases/blood/epidemiology/*virology MH - Time Factors MH - Virus Diseases/blood/epidemiology/*veterinary/virology OTO - NOTNLM OT - Feral pigs OT - Hawaii OT - fluorescence microsphere immunoassay OT - porcine circovirus OT - porcine reproductive and respiratory syndrome virus OT - swine influenza virus EDAT- 2014/11/08 06:00 MHDA- 2015/09/22 06:00 CRDT- 2014/11/08 06:00 PHST- 2014/11/08 06:00 [entrez] PHST- 2014/11/08 06:00 [pubmed] PHST- 2015/09/22 06:00 [medline] AID - 10.7589/2013-09-242 [doi] PST - ppublish SO - J Wildl Dis. 2015 Jan;51(1):239-43. doi: 10.7589/2013-09-242.