PMID- 2538067
OWN - NLM
STAT- MEDLINE
DCOM- 19890414
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 256
IP  - 3 Pt 1
DP  - 1989 Mar
TI  - Regulation of cAMP metabolism by PGE2 in cortical and medullary thick ascending 
      limb of Henle's loop.
PG  - C652-7
AB  - We have examined the regulation by prostaglandin E2 (PGE2) of hormone-induced 
      adenosine 3',5'-cyclic monophosphate (cAMP) accumulation in cells isolated by 
      immunodissection from both the medullary and cortical thick ascending limb of 
      Henle's loop of rabbit kidney. At concentrations greater than 10(-8) M, PGE2, but 
      not sulprostone (16-phenoxy-17,18,19,20-tetranor-PGE2 methylsulfonilamide), 
      caused cAMP accumulation in both cortical and medullary thick limb cells. 
      However, at concentrations of less than or equal to 10(-8) M, both PGE2 and 
      sulprostone inhibited arginine vasopressin (AVP)-, calcitonin-, and 
      glucagon-induced cAMP accumulation in medullary thick ascending limb (mTAL) 
      cells. In cortical thick limb (cTAL) cells, sulprostone also inhibited AVP-, 
      calcitonin-, and parathyroid hormone (PTH)-induced cAMP accumulation. The 
      inhibitory effects of PGE2 and of sulprostone were blocked by pretreatment of 
      mTAL and cTAL cells with pertussis toxin. Membranes prepared from mTAL cells 
      exhibited a [3H]PGE2 binding activity that was stimulated on addition of the 
      stable guanosine 5'-triphosphate (GTP) analogue, 5'-guanosine 
      gamma-thiotriphosphate (GTP gamma S); moreover, sulprostone inhibited [3H]PGE2 
      binding. Our results suggest that PGE2 can function via a prostaglandin E 
      receptor linked to a guanine nucleotide regulatory protein, Gi, to attenuate 
      hormone-induced cAMP formation in both mTAL and cTAL cells of rabbit kidney.
FAU - Nakao, A
AU  - Nakao A
AD  - Department of Biochemistry, Michigan State University, East Lansing 48824.
FAU - Allen, M L
AU  - Allen ML
FAU - Sonnenburg, W K
AU  - Sonnenburg WK
FAU - Smith, W L
AU  - Smith WL
LA  - eng
GR  - DK-22042/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Parathyroid Hormone)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Prostaglandin)
RN  - 0 (Thionucleotides)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 10T9CSU89I (Teriparatide)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 501Q5EQ1GM (sulprostone)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Arginine Vasopressin/pharmacology
MH  - Cyclic AMP/*metabolism
MH  - Dinoprostone/analogs & derivatives/metabolism/*pharmacology
MH  - Guanosine 5'-O-(3-Thiotriphosphate)
MH  - Guanosine Triphosphate/analogs & derivatives/pharmacology
MH  - In Vitro Techniques
MH  - Kidney Cortex/drug effects/*metabolism
MH  - Kidney Medulla/drug effects/*metabolism
MH  - Kidney Tubules/*metabolism
MH  - Kinetics
MH  - Loop of Henle/drug effects/*metabolism
MH  - Parathyroid Hormone/pharmacology
MH  - Peptide Fragments/pharmacology
MH  - Pertussis Toxin
MH  - Rabbits
MH  - Receptors, Prostaglandin/metabolism
MH  - Teriparatide
MH  - Thionucleotides/pharmacology
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
AID - 10.1152/ajpcell.1989.256.3.C652 [doi]
PST - ppublish
SO  - Am J Physiol. 1989 Mar;256(3 Pt 1):C652-7. doi: 10.1152/ajpcell.1989.256.3.C652.