PMID- 25380864
OWN - NLM
STAT- MEDLINE
DCOM- 20151022
LR  - 20181202
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 46
IP  - 8
DP  - 2014 Oct
TI  - Nephroprotective effect of pentoxifylline in renal ischemia-reperfusion in rat 
      depends on the timing of its administration.
PG  - 2555-7
LID - S0041-1345(14)00838-0 [pii]
LID - 10.1016/j.transproceed.2014.09.052 [doi]
AB  - BACKGROUND: Renal ischemia-reperfusion injury (IRI) induces inflammatory reaction 
      damaging kidney. Pentoxifylline (PTX) given before IRI attenuates inflammation 
      and prevents ischemic acute kidney injury (iAKI). Given that in clinical settings 
      IRI is not always predictable, we aimed to assess whether PTX administration 
      during or shortly after IRI affects the course of iAKI in the rat. METHODS: In 58 
      male 10-week-old Sprague-Dawley rats, 14 days after right nephrectomy, a 
      45-minute clamping of solitary renal pedicle was conducted. PTX 100 mg/kg body 
      weight or 0.9% NaCl 1 mL were given subcutaneously either 60 minutes before renal 
      ischemia, 1 minute into ischemia, or 60 minutes after clamp release. Creatinine 
      clearance (ClCr; mL/min/kg body weight), fractional excretions of sodium (FENa 
      [%]) and potassium (FEK [%]), and urine protein/ClCr ratio (Uprot/ClCr [mg/1 mL 
      ClCr]) at 48 hours after IRI were compared between PTX-treated animals and 
      respective controls (Mann-Whitney U test). RESULTS: Kidney function was improved 
      in rats given PTX before IRI compared with controls: ClCr 2.10 +/- 0.44 versus 1.03 
      +/- 0.18; FENa 0.16 +/- 0.12 versus 0.84 +/- 0.55; FEK 40.3 +/- 13.0 versus 75.5 +/- 17.9, 
      respectively (all P < .001). There was no difference in proteinuria: Uprot/ClCr 
      0.004 +/- 0.002 versus 0.004 +/- 0.002. Conversely, the analyzed parameters did not 
      differ between animals administered PTX during IRI and controls: ClCr 0.42 +/- 0.34 
      versus 0.73 +/- 0.43; FENa 2.98 +/- 2.71 versus 3.16 +/- 3.05; FEK 280.1 +/- 155.7 versus 
      206.2 +/- 154.1; and Uprot/ClCr 0.031 +/- 0.029 versus 0.029 +/- 0.031, respectively, 
      nor between rats given PTX after IRI and controls: ClCr 0.29 +/- 0.38 versus 0.40 +/- 
      0.47; FENa 4.25 +/- 3.55 versus 3.80 +/- 3.94; FEK 284.9 +/- 117.5 versus 243.0 +/- 
      150.6; and Uprot/ClCr 0.044 +/- 0.018 versus 0.055 +/- 0.061, respectively. 
      CONCLUSIONS: PTX given only before, and not at the time of renal ischemia or 
      after reperfusion, alleviates subsequent iAKI in the rat. This implicates 
      usefulness of PTX in the clinical settings of expected renal ischemia, like 
      kidney transplantation, and suggests potential benefits of PTX in peritransplant 
      period foremost with donor pretreatment.
FAU - Wystrychowski, W
AU  - Wystrychowski W
AD  - Department of General, Vascular and Transplant Surgery, Medical University of 
      Silesia, Katowice, Poland.
FAU - Wystrychowski, G
AU  - Wystrychowski G
AD  - Department of Internal Medicine, Diabetology, and Nephrology, Medical University 
      of Silesia, Katowice, Poland. Electronic address: wystrych@gmail.com.
FAU - Zukowska-Szczechowska, E
AU  - Zukowska-Szczechowska E
AD  - Department of Internal Medicine, Diabetology, and Nephrology, Medical University 
      of Silesia, Katowice, Poland.
FAU - Obuchowicz, E
AU  - Obuchowicz E
AD  - Department of Pharmacology, Medical University of Silesia, Katowice, Poland.
FAU - Grzeszczak, W
AU  - Grzeszczak W
AD  - Department of Internal Medicine, Diabetology, and Nephrology, Medical University 
      of Silesia, Katowice, Poland.
FAU - Wiecek, A
AU  - Wiecek A
AD  - Department of Nephrology, Endocrinology and Metabolic Diseases, Medical 
      University of Silesia, Katowice, Poland.
FAU - Wystrychowski, A
AU  - Wystrychowski A
AD  - Department of Nephrology, Endocrinology and Metabolic Diseases, Medical 
      University of Silesia, Katowice, Poland.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Free Radical Scavengers)
RN  - SD6QCT3TSU (Pentoxifylline)
SB  - IM
MH  - Acute Kidney Injury/*prevention & control
MH  - Animals
MH  - Constriction
MH  - Free Radical Scavengers/*pharmacology
MH  - Inflammation
MH  - Kidney/*drug effects
MH  - *Kidney Transplantation
MH  - Male
MH  - Nephrectomy
MH  - Pentoxifylline/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*prevention & control
EDAT- 2014/11/09 06:00
MHDA- 2015/10/23 06:00
CRDT- 2014/11/09 06:00
PHST- 2014/11/09 06:00 [entrez]
PHST- 2014/11/09 06:00 [pubmed]
PHST- 2015/10/23 06:00 [medline]
AID - S0041-1345(14)00838-0 [pii]
AID - 10.1016/j.transproceed.2014.09.052 [doi]
PST - ppublish
SO  - Transplant Proc. 2014 Oct;46(8):2555-7. doi: 10.1016/j.transproceed.2014.09.052.