PMID- 2538336
OWN - NLM
STAT- MEDLINE
DCOM- 19890505
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 181
IP  - 2
DP  - 1989 Apr
TI  - Synergistic effect of tumor necrosis factor-alpha and interferon-gamma on 
      collagen synthesis of human skin fibroblasts in vitro.
PG  - 409-19
AB  - The effect of tumor necrosis factor-alpha (TNF alpha) and interferon-gamma (IFN 
      gamma) on collagen metabolism by human diploid fibroblasts in confluent monolayer 
      culture was examined. Recombinant TNF alpha reduced collagen mRNA levels 2-fold 
      and stimulated collagenase mRNA levels 5-fold, while recombinant IFN gamma 
      affected only collagen mRNA levels. The combination of TNF alpha (10 ng/ml) and 
      IFN gamma (100 ng/ml) resulted in a much stronger (about 30-fold) reduction of 
      collagen mRNA levels indicating that the two cytokines act synergistically. In 
      contrast no such synergism was observed with respect to collagenase mRNA levels. 
      The effect of TNF alpha and IFN gamma on collagen metabolism reported here 
      indicates a complex interaction of different cytokines in the control of tissue 
      remodeling that occurs during inflammation, repair, or atrophy.
FAU - Scharffetter, K
AU  - Scharffetter K
AD  - Dermatologische Klinik, Ludwig Maximilians Universitat, Munchen, Federal Republic 
      of Germany.
FAU - Heckmann, M
AU  - Heckmann M
FAU - Hatamochi, A
AU  - Hatamochi A
FAU - Mauch, C
AU  - Mauch C
FAU - Stein, B
AU  - Stein B
FAU - Riethmuller, G
AU  - Riethmuller G
FAU - Ziegler-Heitbrock, H W
AU  - Ziegler-Heitbrock HW
FAU - Krieg, T
AU  - Krieg T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.3 (Microbial Collagenase)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Collagen/*biosynthesis/genetics
MH  - Drug Synergism
MH  - Fibroblasts
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Microbial Collagenase/genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1989/04/01 00:00
MHDA- 1989/04/01 00:01
CRDT- 1989/04/01 00:00
PHST- 1989/04/01 00:00 [pubmed]
PHST- 1989/04/01 00:01 [medline]
PHST- 1989/04/01 00:00 [entrez]
AID - 10.1016/0014-4827(89)90098-0 [doi]
PST - ppublish
SO  - Exp Cell Res. 1989 Apr;181(2):409-19. doi: 10.1016/0014-4827(89)90098-0.