PMID- 25384086
OWN - NLM
STAT- MEDLINE
DCOM- 20150210
LR  - 20181113
IS  - 1546-170X (Electronic)
IS  - 1078-8956 (Print)
IS  - 1078-8956 (Linking)
VI  - 20
IP  - 12
DP  - 2014 Dec
TI  - Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph 
      nodes.
PG  - 1458-63
LID - 10.1038/nm.3709 [doi]
AB  - Antibodies are critical for defense against a variety of microbes, but they may 
      also be pathogenic in some autoimmune diseases. Many effector functions of 
      antibodies are mediated by Fcgamma receptors (FcgammaRs), which are found on most immune 
      cells, including dendritic cells (DCs)-important antigen-presenting cells that 
      play a central role in inducing antigen-specific tolerance or immunity. Following 
      antigen acquisition in peripheral tissues, DCs migrate to draining lymph nodes 
      via the lymphatics to present antigen to T cells. Here we demonstrate that FcgammaR 
      engagement by IgG immune complexes (ICs) stimulates DC migration from peripheral 
      tissues to the paracortex of draining lymph nodes. In vitro, IC-stimulated mouse 
      and human DCs showed greater directional migration in a chemokine (C-C) ligand 19 
      (CCL19) gradient and increased chemokine (C-C) receptor 7 (CCR7) expression. 
      Using intravital two-photon microscopy, we observed that local administration of 
      IC resulted in dermal DC mobilization. We confirmed that dermal DC migration to 
      lymph nodes depended on CCR7 and increased in the absence of the inhibitory 
      receptor FcgammaRIIB. These observations have relevance to autoimmunity because 
      autoantibody-containing serum from humans with systemic lupus erythematosus (SLE) 
      and from a mouse model of SLE also increased dermal DC migration in vivo, 
      suggesting that this process may occur in lupus, potentially driving the 
      inappropriate localization of autoantigen-bearing DCs.
FAU - Clatworthy, Menna R
AU  - Clatworthy MR
AD  - 1] Department of Medicine, MRC Laboratory of Molecular Biology, University of 
      Cambridge, Cambridge, UK. [2] Laboratory of Systems Biology, Lymphocyte Biology 
      Section, National Institute of Allergy and Infectious Diseases, National 
      Institutes of Health, Bethesda, Maryland, USA.
FAU - Aronin, Caren E Petrie
AU  - Aronin CE
AD  - Laboratory of Systems Biology, Lymphocyte Biology Section, National Institute of 
      Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 
      Maryland, USA.
FAU - Mathews, Rebeccah J
AU  - Mathews RJ
AD  - Department of Medicine, MRC Laboratory of Molecular Biology, University of 
      Cambridge, Cambridge, UK.
FAU - Morgan, Nicole Y
AU  - Morgan NY
AD  - Biomedical Engineering and Physical Sciences Resource, Microfabrication and 
      Microfluidics Unit, National Institute of Biomedical Imaging and Bioengineering, 
      National Institutes of Health, Bethesda, Maryland, USA.
FAU - Smith, Kenneth G C
AU  - Smith KG
AD  - Cambridge Institute for Medical Research and Department of Medicine, University 
      of Cambridge School of Clinical Medicine, Cambridge, UK.
FAU - Germain, Ronald N
AU  - Germain RN
AD  - Laboratory of Systems Biology, Lymphocyte Biology Section, National Institute of 
      Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 
      Maryland, USA.
LA  - eng
GR  - 081020/Wellcome Trust/United Kingdom
GR  - 100140/Wellcome Trust/United Kingdom
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20141110
PL  - United States
TA  - Nat Med
JT  - Nature medicine
JID - 9502015
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Chemokine CCL19)
RN  - 0 (Fc gamma receptor IIB)
RN  - 0 (Receptors, CCR7)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Animals
MH  - Antigen-Antibody Complex/*immunology
MH  - Cell Movement/*immunology
MH  - Chemokine CCL19/immunology
MH  - Dendritic Cells/immunology
MH  - Humans
MH  - Langerhans Cells/*immunology
MH  - Lupus Erythematosus, Systemic/*immunology
MH  - Lymph Nodes/*immunology
MH  - Mice
MH  - Receptors, CCR7/*immunology
MH  - Receptors, IgG/*immunology
PMC - PMC4283039
MID - EMS60150
OID - NLM: EMS60150
EDAT- 2014/11/11 06:00
MHDA- 2015/02/11 06:00
PMCR- 2015/06/01
CRDT- 2014/11/11 06:00
PHST- 2014/07/13 00:00 [received]
PHST- 2014/08/25 00:00 [accepted]
PHST- 2014/11/11 06:00 [entrez]
PHST- 2014/11/11 06:00 [pubmed]
PHST- 2015/02/11 06:00 [medline]
PHST- 2015/06/01 00:00 [pmc-release]
AID - nm.3709 [pii]
AID - 10.1038/nm.3709 [doi]
PST - ppublish
SO  - Nat Med. 2014 Dec;20(12):1458-63. doi: 10.1038/nm.3709. Epub 2014 Nov 10.