PMID- 25385443
OWN - NLM
STAT- MEDLINE
DCOM- 20150911
LR  - 20171116
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 55
IP  - 6 Pt 2
DP  - 2015 Jun
TI  - Impact of priming on the response of neutrophils to human neutrophil 
      alloantigen-3a antibodies.
PG  - 1512-21
LID - 10.1111/trf.12898 [doi]
AB  - BACKGROUND: Human neutrophil alloantigen-3a (HNA-3a) antibodies can induce 
      transfusion-related acute lung injury (TRALI). The severity of TRALI varies 
      largely among the affected patients. Severe comorbidity seems to increase the 
      susceptibility for TRALI, potentially by priming of neutrophils. Thus, the impact 
      of neutrophil priming on HNA-3a antibody-mediated neutrophil aggregation and 
      CD11b surface expression was investigated. STUDY DESIGN AND METHODS: Neutrophils 
      were primed using formyl-methionyl-leucyl-phenylalanine (fMLP) or bacterial 
      lipopolysaccharide (LPS). Granulocyte aggregation and CD11b surface expression 
      were evaluated by the granulocyte agglutination test and by flow cytometry (FC), 
      respectively. Priming-induced changes in the surface expression of choline 
      transporter-like protein 2 (CTL2) and the CTL2 mRNA expression were assessed by 
      FC and quantitative real-time polymerase chain reaction, respectively. RESULTS: 
      Priming of neutrophils lowered the amount of HNA-3a antibodies required for 
      inducing granulocyte aggregation in a dose-dependent manner by 50% to 75%. The 
      priming agent concentration necessary for this response differed between donors. 
      Priming slightly enhanced binding of HNA-3a antibodies to neutrophils. However, 
      CTL2 de novo synthesis was not induced after priming with LPS, indicating that 
      increased HNA-3a antibody binding was likely caused by translocation of 
      intracellular CTL2 to the surface or by increased affinity of HNA-3a antibodies 
      to CTL2. HNA-3a antibodies influenced CD11b surface expression on neutrophils 
      only marginally, which was also not potentiated by priming with fMLP or LPS. 
      CONCLUSION: This study provides experimental evidence supporting the "threshold 
      model" of TRALI. Priming of neutrophils with fMLP or LPS increases their 
      aggregation response to HNA-3a antibodies by lowering the required antibody 
      amount.
CI  - (c) 2014 AABB.
FAU - Berthold, Tom
AU  - Berthold T
AD  - Department of Transfusion Medicine, Institute for Immunology and Transfusion 
      Medicine, Greifswald, Germany.
AD  - Department of Functional Genomics, Interfaculty Institute for Genetics and 
      Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
FAU - Muschter, Stefan
AU  - Muschter S
AD  - Department of Transfusion Medicine, Institute for Immunology and Transfusion 
      Medicine, Greifswald, Germany.
AD  - Department of Functional Genomics, Interfaculty Institute for Genetics and 
      Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
FAU - Schubert, Nicole
AU  - Schubert N
AD  - Department of Transfusion Medicine, Institute for Immunology and Transfusion 
      Medicine, Greifswald, Germany.
FAU - Wesche, Jan
AU  - Wesche J
AD  - Department of Transfusion Medicine, Institute for Immunology and Transfusion 
      Medicine, Greifswald, Germany.
FAU - Ameling, Sabine
AU  - Ameling S
AD  - Department of Functional Genomics, Interfaculty Institute for Genetics and 
      Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
FAU - Teumer, Alexander
AU  - Teumer A
AD  - Department of Functional Genomics, Interfaculty Institute for Genetics and 
      Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
FAU - Reil, Angelika
AU  - Reil A
AD  - German Red Cross Blood Donation Service West, Hagen, Germany.
FAU - Bux, Jurgen
AU  - Bux J
AD  - German Red Cross Blood Donation Service West, Hagen, Germany.
FAU - Bakchoul, Tamam
AU  - Bakchoul T
AD  - Department of Transfusion Medicine, Institute for Immunology and Transfusion 
      Medicine, Greifswald, Germany.
FAU - Greinacher, Andreas
AU  - Greinacher A
AD  - Department of Transfusion Medicine, Institute for Immunology and Transfusion 
      Medicine, Greifswald, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141111
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (3a alloantigen, human)
RN  - 0 (Antigens, Human Platelet)
RN  - 0 (CD11b Antigen)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (SLC44A2 protein, human)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Agglutination Tests
MH  - Antigen Presentation/*immunology
MH  - Antigens, Human Platelet/*immunology/pharmacology
MH  - CD11b Antigen/metabolism
MH  - Cell Aggregation/immunology
MH  - Cells, Cultured
MH  - Granulocytes/immunology
MH  - Humans
MH  - Immunologic Memory/drug effects/*physiology
MH  - Lipopolysaccharides/immunology/pharmacology
MH  - Membrane Glycoproteins/genetics/metabolism
MH  - Membrane Transport Proteins/genetics/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/immunology/pharmacology
MH  - Neutrophil Activation/drug effects/immunology
MH  - Neutrophils/drug effects/*immunology
EDAT- 2014/11/12 06:00
MHDA- 2015/09/12 06:00
CRDT- 2014/11/12 06:00
PHST- 2014/04/24 00:00 [received]
PHST- 2014/08/20 00:00 [revised]
PHST- 2014/08/24 00:00 [accepted]
PHST- 2014/11/12 06:00 [entrez]
PHST- 2014/11/12 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
AID - 10.1111/trf.12898 [doi]
PST - ppublish
SO  - Transfusion. 2015 Jun;55(6 Pt 2):1512-21. doi: 10.1111/trf.12898. Epub 2014 Nov 
      11.