PMID- 25386077
OWN - NLM
STAT- MEDLINE
DCOM- 20150810
LR  - 20211203
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 20
IP  - 41
DP  - 2014 Nov 7
TI  - N-acetylcysteine attenuates reactive-oxygen-species-mediated endoplasmic 
      reticulum stress during liver ischemia-reperfusion injury.
PG  - 15289-98
LID - 10.3748/wjg.v20.i41.15289 [doi]
AB  - AIM: To investigate the effects of N-acetylcysteine (NAC) on endoplasmic 
      reticulum (ER) stress and tissue injury during liver ischemia reperfusion injury 
      (IRI). METHODS: Mice were injected with NAC (300 mg/kg) intraperitoneally 2 h 
      before ischemia. Real-time polymerase chain reaction and western blotting 
      determined ER stress molecules (GRP78, ATF4 and CHOP). To analyze the role of NAC 
      in reactive oxygen species (ROS)-mediated ER stress and apoptosis, lactate 
      dehydrogenase (LDH) was examined in cultured hepatocytes treated by H2O2 or 
      thapsigargin (TG). RESULTS: NAC treatment significantly reduced the level of ROS 
      and attenuated ROS-induced liver injury after IRI, based on glutathione, 
      malondialdehyde, serum alanine aminotransferase levels, and histopathology. 
      ROS-mediated ER stress was significantly inhibited in NAC-treated mice. In 
      addition, NAC treatment significantly reduced caspase-3 activity and apoptosis 
      after reperfusion, which correlated with the protein expression of Bcl-2 and 
      Bcl-xl. Similarly, NAC treatment significantly inhibited LDH release from 
      hepatocytes treated by H2O2 or TG. CONCLUSION: This study provides new evidence 
      for the protective effects of NAC treatment on hepatocytes during IRI. Through 
      inhibition of ROS-mediated ER stress, NAC may be critical to inhibit the 
      ER-stress-related apoptosis pathway.
FAU - Sun, Yong
AU  - Sun Y
AD  - Yong Sun, Department of General Surgery, Huai'an First People's Hospital, Nanjing 
      Medical University, Huai'an 223300, Jiangsu Province, China.
FAU - Pu, Li-Yong
AU  - Pu LY
AD  - Yong Sun, Department of General Surgery, Huai'an First People's Hospital, Nanjing 
      Medical University, Huai'an 223300, Jiangsu Province, China.
FAU - Lu, Ling
AU  - Lu L
AD  - Yong Sun, Department of General Surgery, Huai'an First People's Hospital, Nanjing 
      Medical University, Huai'an 223300, Jiangsu Province, China.
FAU - Wang, Xue-Hao
AU  - Wang XH
AD  - Yong Sun, Department of General Surgery, Huai'an First People's Hospital, Nanjing 
      Medical University, Huai'an 223300, Jiangsu Province, China.
FAU - Zhang, Feng
AU  - Zhang F
AD  - Yong Sun, Department of General Surgery, Huai'an First People's Hospital, Nanjing 
      Medical University, Huai'an 223300, Jiangsu Province, China.
FAU - Rao, Jian-Hua
AU  - Rao JH
AD  - Yong Sun, Department of General Surgery, Huai'an First People's Hospital, Nanjing 
      Medical University, Huai'an 223300, Jiangsu Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Antioxidants)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Hspa5 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*pharmacology
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cells, Cultured
MH  - Cytoprotection
MH  - Disease Models, Animal
MH  - Endoplasmic Reticulum/*drug effects/metabolism/pathology
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoplasmic Reticulum Stress/*drug effects
MH  - Liver/*blood supply/*drug effects/metabolism/pathology
MH  - Liver Diseases/*drug therapy/metabolism/pathology
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Oxidative Stress/*drug effects
MH  - Reactive Oxygen Species/*metabolism
MH  - Reperfusion Injury/*drug therapy/metabolism/pathology
MH  - Signal Transduction/drug effects
PMC - PMC4223262
OTO - NOTNLM
OT  - Apoptosis
OT  - Endoplasmic reticulum stress
OT  - Liver ischemia-reperfusion
OT  - N-acetylcysteine
OT  - Reactive oxygen species
EDAT- 2014/11/12 06:00
MHDA- 2015/08/11 06:00
PMCR- 2014/11/07
CRDT- 2014/11/12 06:00
PHST- 2013/02/11 00:00 [received]
PHST- 2014/04/08 00:00 [revised]
PHST- 2014/06/26 00:00 [accepted]
PHST- 2014/11/12 06:00 [entrez]
PHST- 2014/11/12 06:00 [pubmed]
PHST- 2015/08/11 06:00 [medline]
PHST- 2014/11/07 00:00 [pmc-release]
AID - 10.3748/wjg.v20.i41.15289 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2014 Nov 7;20(41):15289-98. doi: 
      10.3748/wjg.v20.i41.15289.