PMID- 2538828
OWN - NLM
STAT- MEDLINE
DCOM- 19890428
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 86
IP  - 6
DP  - 1989 Mar
TI  - Hepatitis B virus X gene can transactivate heterologous viral sequences.
PG  - 2046-50
AB  - The smallest open reading frame of hepatitis B virus (HBV) has been designated 
      the X gene and its biological function during HBV infection and replication is 
      not known. Experiments described here demonstrate that expression of the HBV X 
      gene in HepG2 cells containing a plasmid with the chloramphenicol 
      acetyltransferase (CAT) gene under control of the human immunodeficiency virus 
      (HIV-1) long terminal repeat (LTR) sequence leads to a marked increase in CAT 
      gene transcription as well as expression of the gene product (CAT). The HIV-1 
      tatIII gene and the HBV X gene together increased HIV-1 LTR-regulated CAT 
      expression above that observed with either gene alone, suggesting a synergistic 
      effect of the X gene and tat. HBV X gene also stimulated expression of the CAT 
      gene under control of the simian virus 40 enhancer and early promoter but not the 
      visna virus LTR or the human T-cell lymphotropic virus type I (HTLV-I) LTR, 
      indicating that the HBV X gene can transactivate some but not other heterologous 
      viral sequences. Transactivation of the HIV-1 LTR by the HBV X gene varied in 
      different cell lines, suggesting that it may be mediated by a cellular factor(s).
FAU - Twu, J S
AU  - Twu JS
AD  - Division of Infectious Diseases, Stanford University School of Medicine, CA 
      94305-5107.
FAU - Robinson, W S
AU  - Robinson WS
LA  - eng
GR  - AI-13526/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA, Recombinant)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Cell Line
MH  - Chloramphenicol O-Acetyltransferase/genetics
MH  - DNA, Recombinant
MH  - Dexamethasone/pharmacology
MH  - Enhancer Elements, Genetic
MH  - *Gene Expression Regulation/drug effects
MH  - *Genes, Viral
MH  - HIV/genetics
MH  - Hepatitis B virus/*genetics
MH  - Plasmids
MH  - Promoter Regions, Genetic
MH  - Repetitive Sequences, Nucleic Acid
MH  - Simian virus 40/genetics
MH  - Species Specificity
MH  - Transcription, Genetic
MH  - Transfection
MH  - Visna-maedi virus/genetics
PMC - PMC286844
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
PMCR- 1989/09/01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
PHST- 1989/09/01 00:00 [pmc-release]
AID - 10.1073/pnas.86.6.2046 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1989 Mar;86(6):2046-50. doi: 10.1073/pnas.86.6.2046.