PMID- 25388873 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20160726 LR - 20181113 IS - 0920-9069 (Print) IS - 1573-0778 (Electronic) IS - 0920-9069 (Linking) VI - 68 IP - 4 DP - 2016 Aug TI - Comparative cytogenetic analysis of marine needlefishes (Beloniformes) from southern Brazil. PG - 1585-9 LID - 10.1007/s10616-014-9809-x [doi] AB - Cytogenetic studies have assisted in the taxonomic classification of organisms, especially those involving species with highly similar morphologic characteristics, or so-called cryptic species. Strongylura marina and Strongylura timucu collected from Paranagua Bay, Parana Coast in Southern Brazil are considered cryptic species, and the identification of interspecific variations based on the number and/or morphology of its chromosomes may serve as differentiating cytotaxonomic markers. Chromosomes of the two species were subjected to different banding and staining methods (C-, Ag-, and DAPI-CMA3), as well as chromosomal mapping of major rDNA (45S), revealed with an 18S probe by fluorescence in situ hybridization (FISH). The pattern of distribution of constitutive heterochromatin showed distinct features involving the pericentromeric and telomeric bands in both species. In S. marina, chromosome 1 represents the main species-specific marker, appearing almost entirely heterochromatic. In both species, the 45S rDNA is located at terminal region of the short arm of the chromosome 6, as detected by silver nitrate staining and FISH. Despite the apparent conserved diploid number of 48 chromosomes, data on the karyotype microstructure characterize the cytogenetic profile of the genus and may allow the establishment of cytotaxonomic and evolutionary inferences for these fishes. FAU - Cipriano, Roger Raupp AU - Cipriano RR AD - Departamento de Fitotecnia e Fitossanitarismo, Setor de Ciencias Agrarias, Universidade Federal do Parana, Rua dos Funcionarios, 1540, Curitiba, Parana, CEP: 80035-050, Brazil. rogerraupp@gmail.com. FAU - Noleto, Rafael Bueno AU - Noleto RB AD - Departamento de Biologia, Universidade Estadual do Parana, Campus Uniao da Vitoria, Praca Coronel Amazonas, Uniao da Vitoria, Parana, CEP: 84600-000, Brazil. rafanoleto@yahoo.com.br. FAU - Kantek, Daniel Luis Zanella AU - Kantek DL AD - Taiama Ecological Station, Instituto Chico Mendes de Conservacao da Biodiversidade, Caceres, Mato Grosso, Brazil. FAU - da Silva Cortinhas, Maria Cristina AU - da Silva Cortinhas MC AD - Laboratorio de Crustaceos Decapodes, Instituto de Oceanografia, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil. FAU - Cestari, Marta Margarete AU - Cestari MM AD - Departamento de Genetica, Universidade Federal do Parana, Centro Politecnico, Curitiba, Parana, CEP: 81530-900, Brazil. LA - eng PT - Journal Article DEP - 20141112 PL - United States TA - Cytotechnology JT - Cytotechnology JID - 8807027 PMC - PMC4960110 OTO - NOTNLM OT - Ag-NORs OT - C-band OT - FISH OT - Karyotype OT - Marine teleosts EDAT- 2014/11/13 06:00 MHDA- 2014/11/13 06:01 PMCR- 2017/08/01 CRDT- 2014/11/13 06:00 PHST- 2014/05/18 00:00 [received] PHST- 2014/10/27 00:00 [accepted] PHST- 2014/11/13 06:00 [entrez] PHST- 2014/11/13 06:00 [pubmed] PHST- 2014/11/13 06:01 [medline] PHST- 2017/08/01 00:00 [pmc-release] AID - 10.1007/s10616-014-9809-x [pii] AID - 9809 [pii] AID - 10.1007/s10616-014-9809-x [doi] PST - ppublish SO - Cytotechnology. 2016 Aug;68(4):1585-9. doi: 10.1007/s10616-014-9809-x. Epub 2014 Nov 12.