PMID- 25389708
OWN - NLM
STAT- MEDLINE
DCOM- 20150626
LR  - 20181202
IS  - 1532-0987 (Electronic)
IS  - 0891-3668 (Linking)
VI  - 33
IP  - 12
DP  - 2014 Dec
TI  - Decreased toll-like receptor-4/myeloid differentiation factor 88 response leads 
      to defective interleukin-1beta production in term low birth weight newborns.
PG  - 1270-6
LID - 10.1097/INF.0000000000000416 [doi]
AB  - BACKGROUND: Morbidity and mortality rates are very high in low birth weight (LBW) 
      newborns because of their increased susceptibility to infections compared with 
      normal birth weight (NBW) newborns. A case and control study was designed to 
      identify the status of toll-like receptor-4 (TLR-4) signaling and maternally 
      derived immunoglobulin-G (IgG) subclasses in term LBW newborns compared with NBW 
      newborns. METHODS: To understand the basis of increased susceptibility to 
      infections in LBW newborns, the levels of pro- and antiinflammatory cytokines 
      interleukin-1beta (IL-1beta) and interleukin-10 (IL-10), respectively, released in 
      response to lipopolysaccharide (LPS) stimulation of cord blood cells of LBW (n = 
      20) and NBW (n = 18) newborns, were quantified by enzyme-linked immunosorbent 
      assay. Further, LPS-induced expression of TLR-4 and basal and LPS-induced 
      expression of myeloid differentiation factor 88 (MyD88) were examined at mRNA 
      levels in both groups. The levels of IgG subclasses in LBW (n = 20) and NBW (n = 
      18) newborns were quantified by enzyme-linked immunosorbent assay to explore the 
      role of maternally derived immunity in LBW newborns. RESULTS: LPS-mediated 
      release of IL-1beta was significantly diminished in LBW newborns when compared with 
      NBW newborns, whereas there was no significant difference in IL-10. Decreased 
      production of IL-1beta in LBW newborns was correlated with reduced expression of 
      TLR-4 and MyD88 mRNA. No significant differences were observed in the levels of 
      all 4 IgG subclasses between LBW and NBW newborns. CONCLUSIONS: Decreased 
      production of IL-1beta in LBW newborns was correlated with reduced expression of 
      TLR-4 and MyD88 mRNA. This raises the possibility of increased susceptibility to 
      infections in LBW when compared with the NBW newborns at term. Comparable levels 
      of IgG subclasses in the 2 groups of newborns indicate that IgG is not a limiting 
      factor in defense against infection in LBW newborns.
FAU - Singh, Vikas Vikram
AU  - Singh VV
AD  - From the *Department of Molecular and Human Genetics, Faculty of Science; and 
      daggerDepartment of Pediatrics, Institute of Medical Sciences, Banaras Hindu 
      University, Varanasi, India.
FAU - Chauhan, Sudhir Kumar
AU  - Chauhan SK
FAU - Rai, Richa
AU  - Rai R
FAU - Kumar, Ashok
AU  - Kumar A
FAU - Rai, Geeta
AU  - Rai G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pediatr Infect Dis J
JT  - The Pediatric infectious disease journal
JID - 8701858
RN  - 0 (IL10 protein, human)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MYD88 protein, human)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Communicable Diseases/immunology
MH  - Disease Susceptibility
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fetal Blood/immunology
MH  - Gene Expression Profiling
MH  - Humans
MH  - Immunity, Maternally-Acquired
MH  - Immunoglobulin G/blood
MH  - *Infant, Low Birth Weight
MH  - Infant, Newborn
MH  - Interleukin-10/metabolism
MH  - Interleukin-1beta/*metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - Lipopolysaccharides/immunology
MH  - Male
MH  - Myeloid Differentiation Factor 88/*biosynthesis/genetics
MH  - Pregnancy
MH  - Toll-Like Receptor 4/*biosynthesis/genetics
EDAT- 2014/11/13 06:00
MHDA- 2015/06/27 06:00
CRDT- 2014/11/13 06:00
PHST- 2014/11/13 06:00 [entrez]
PHST- 2014/11/13 06:00 [pubmed]
PHST- 2015/06/27 06:00 [medline]
AID - 00006454-201412000-00015 [pii]
AID - 10.1097/INF.0000000000000416 [doi]
PST - ppublish
SO  - Pediatr Infect Dis J. 2014 Dec;33(12):1270-6. doi: 10.1097/INF.0000000000000416.