PMID- 25390472
OWN - NLM
STAT- MEDLINE
DCOM- 20160204
LR  - 20181202
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 24
IP  - 7
DP  - 2015 Apr 1
TI  - Mesenchymal stem cells support neuronal fiber growth in an organotypic brain 
      slice co-culture model.
PG  - 824-35
LID - 10.1089/scd.2014.0262 [doi]
AB  - Mesenchymal stem cells (MSCs) have been identified as promising candidates for 
      neuroregenerative cell therapies. However, the impact of different isolation 
      procedures on the functional and regenerative characteristics of MSC populations 
      has not been studied thoroughly. To quantify these differences, we directly 
      compared classically isolated bulk bone marrow-derived MSCs (bulk BM-MSCs) to the 
      subpopulation Sca-1(+)Lin(-)CD45(-)-derived MSCs(-) (SL45-MSCs), isolated by 
      fluorescence-activated cell sorting from bulk BM-cell suspensions. Both 
      populations were analyzed with respect to functional readouts, that are, 
      frequency of fibroblast colony forming units (CFU-f), general morphology, and 
      expression of stem cell markers. The SL45-MSC population is characterized by 
      greater morphological homogeneity, higher CFU-f frequency, and significantly 
      increased nestin expression compared with bulk BM-MSCs. We further quantified the 
      potential of both cell populations to enhance neuronal fiber growth, using an ex 
      vivo model of organotypic brain slice co-cultures of the mesocortical 
      dopaminergic projection system. The MSC populations were cultivated underneath 
      the slice co-cultures without direct contact using a transwell system. After 
      cultivation, the fiber density in the border region between the two brain slices 
      was quantified. While both populations significantly enhanced fiber outgrowth as 
      compared with controls, purified SL45-MSCs stimulated fiber growth to a larger 
      degree. Subsequently, we analyzed the expression of different growth factors in 
      both cell populations. The results show a significantly higher expression of 
      brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor in 
      the SL45-MSCs population. Altogether, we conclude that MSC preparations enriched 
      for primary MSCs promote neuronal regeneration and axonal regrowth, more 
      effectively than bulk BM-MSCs, an effect that may be mediated by a higher BDNF 
      secretion.
FAU - Sygnecka, Katja
AU  - Sygnecka K
AD  - 1 Translational Centre for Regenerative Medicine (TRM), University of Leipzig , 
      Leipzig, Germany .
FAU - Heider, Andreas
AU  - Heider A
FAU - Scherf, Nico
AU  - Scherf N
FAU - Alt, Rudiger
AU  - Alt R
FAU - Franke, Heike
AU  - Franke H
FAU - Heine, Claudia
AU  - Heine C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141217
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Nestin)
SB  - IM
MH  - Animals
MH  - Brain/*cytology/drug effects
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Coculture Techniques
MH  - Culture Media, Conditioned/*pharmacology
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Fibers/*drug effects/metabolism/physiology
MH  - *Nerve Regeneration
MH  - Nestin/genetics/metabolism
EDAT- 2014/11/13 06:00
MHDA- 2016/02/05 06:00
CRDT- 2014/11/13 06:00
PHST- 2014/11/13 06:00 [entrez]
PHST- 2014/11/13 06:00 [pubmed]
PHST- 2016/02/05 06:00 [medline]
AID - 10.1089/scd.2014.0262 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2015 Apr 1;24(7):824-35. doi: 10.1089/scd.2014.0262. Epub 2014 
      Dec 17.