PMID- 25392979
OWN - NLM
STAT- MEDLINE
DCOM- 20160815
LR  - 20190910
IS  - 1522-2594 (Electronic)
IS  - 0740-3194 (Print)
IS  - 0740-3194 (Linking)
VI  - 74
IP  - 5
DP  - 2015 Nov
TI  - In vivo proton MR spectroscopy of pancreatic neuroendocrine tumors in a multiple 
      endocrine neoplasia type 1 conditional knockout mouse model.
PG  - 1221-6
LID - 10.1002/mrm.25529 [doi]
AB  - PURPOSE: MR spectroscopy (MRS) can improve diagnosis and follow treatment in 
      cancer. However, no study has yet reported application of in vivo (1)H-MRS in 
      malignant pancreatic lesions. This study quantitatively determined whether in 
      vivo (1)H-MRS on multiple endocrine neoplasia type 1 (Men1) conditional knockout 
      (KO) mice and their wild type (WT) littermates could detect differences in total 
      choline (tCho) levels between tumor and control pancreas. METHODS: Relative tCho 
      levels in pancreatic tumors or pancreata from KO and WT mice were determined 
      using in vivo (1)H-MRS at 9.4 T. The levels of Cho-containing compounds were also 
      quantified using in vitro (1)H-NMR on extracts of pancreatic tissues from KO and 
      WT mice, respectively, and on extracts of pancreatic tissues from patients with 
      pancreatic neuroendocrine tumors (PNETs). RESULTS: tCho levels measured by in 
      vivo (1)H-MRS were significantly higher in PNETs from KO mice compared to the 
      normal pancreas from WT mice. The elevated choline-containing compounds were also 
      identified in pancreatic tumors from KO mice and tissues from patients with PNETs 
      via in vitro (1)H-NMR. CONCLUSION: These results indicate the potential use of 
      tCho levels estimated via in vivo (1)H-MRS in differentiating malignant 
      pancreatic tumors from benign tumors.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Cui, Min-Hui
AU  - Cui MH
AD  - Gruss Magnetic Resonance Research Center, Albert Einstein College of Medicine, 
      Bronx, New York, USA.
AD  - Department of Radiology, Albert Einstein College of Medicine, Bronx, New York, 
      USA.
AD  - Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, 
      USA.
FAU - Branch, Craig A
AU  - Branch CA
AD  - Gruss Magnetic Resonance Research Center, Albert Einstein College of Medicine, 
      Bronx, New York, USA.
AD  - Department of Radiology, Albert Einstein College of Medicine, Bronx, New York, 
      USA.
AD  - Department of Physiology and Biophysics, Albert Einstein College of Medicine, 
      Bronx, New York, USA.
FAU - Cahill, Sean M
AU  - Cahill SM
AD  - Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, 
      USA.
FAU - Quinn, Thomas J
AU  - Quinn TJ
AD  - Department of Surgery, Albert Einstein College of Medicine, Bronx, New York, USA.
FAU - Adem, Asha
AU  - Adem A
AD  - Department of Surgery, Albert Einstein College of Medicine, Bronx, New York, USA.
FAU - Libutti, Steven K
AU  - Libutti SK
AD  - Department of Surgery, Albert Einstein College of Medicine, Bronx, New York, USA.
AD  - Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, 
      USA.
FAU - Yuan, Ziqiang
AU  - Yuan Z
AD  - Department of Surgery, Albert Einstein College of Medicine, Bronx, New York, USA.
LA  - eng
GR  - U54CA151668/CA/NCI NIH HHS/United States
GR  - P30 CA013330/CA/NCI NIH HHS/United States
GR  - UL1 TR001073/TR/NCATS NIH HHS/United States
GR  - U54 CA151668/CA/NCI NIH HHS/United States
GR  - P50 CA174521/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20141113
PL  - United States
TA  - Magn Reson Med
JT  - Magnetic resonance in medicine
JID - 8505245
RN  - 0 (Transcription Factors)
RN  - N91BDP6H0X (Choline)
SB  - IM
MH  - Animals
MH  - Choline/analysis/chemistry/metabolism
MH  - Female
MH  - Magnetic Resonance Imaging/*methods
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Multiple Endocrine Neoplasia Type 1/*chemistry/*diagnosis/pathology
MH  - Pancreatic Neoplasms/*chemistry/*diagnosis/pathology
MH  - Transcription Factors/genetics
PMC - PMC4430461
MID - NIHMS646263
OTO - NOTNLM
OT  - Men1 conditional knockout mouse model
OT  - in vivo proton MR spectroscopy
OT  - pancreatic neuroendocrine tumors
OT  - total choline levels
EDAT- 2014/11/14 06:00
MHDA- 2016/08/16 06:00
PMCR- 2016/11/01
CRDT- 2014/11/14 06:00
PHST- 2014/09/18 00:00 [received]
PHST- 2014/10/20 00:00 [revised]
PHST- 2014/10/24 00:00 [accepted]
PHST- 2014/11/14 06:00 [entrez]
PHST- 2014/11/14 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
PHST- 2016/11/01 00:00 [pmc-release]
AID - 10.1002/mrm.25529 [doi]
PST - ppublish
SO  - Magn Reson Med. 2015 Nov;74(5):1221-6. doi: 10.1002/mrm.25529. Epub 2014 Nov 13.