PMID- 25393344
OWN - NLM
STAT- MEDLINE
DCOM- 20151120
LR  - 20220408
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 43
IP  - 4
DP  - 2014 Dec
TI  - Preserving whole blood in formalin extends the specimen stability period for 
      manual cell counts for fish.
PG  - 613-20
LID - 10.1111/vcp.12214 [doi]
AB  - BACKGROUND: Total blood cell counts for fish are challenging, especially due to 
      cell fragility. Thrombocyte aggregation, cell distortion, and lysis can occur 
      within hours of collection; therefore, hemocytometer counts may not be possible 
      for large sample numbers. Preserving whole blood in formalin offers a simple way 
      to extend the specimen stability period for cell counts. OBJECTIVES: The 
      objectives of this study were to characterize and compare the analytical 
      performance of the Natt-Herrick method for fish blood cell counts using freshly 
      collected and formalin-fixed specimens. METHODS: Specimens from 11 elasmobranch 
      species (n = 36) were compared for WBC counts. WBC, RBC, and thrombocyte counts 
      from 50 striped bass, Morone saxatilis, were compared including 2 time points for 
      the formalin-preserved cells (appr. 1-week and 1-month storage). Coefficient of 
      variation (CV), bias, and total error (TEcalc ) were calculated. TEcalc was 
      compared with allowable total error (TEa ) defined by the Clinical Laboratory 
      Improvements Amendment (CLIA) for human blood cell counts. RESULTS: The CV and 
      TEcalc for fresh and fixed WBC and thrombocyte counts met CLIA limits. In both 
      the fresh and preserved cells, the RBC CV and TEcalc were nearly 3 times higher 
      than the TEa . CONCLUSIONS: Preserving freshly collected blood in 10% formalin is 
      a reliable method to maintain cell morphology for manual counts for up to 1 month 
      post collection. This is especially useful for field studies, where laboratory 
      access is limited. Further evaluation is needed to determine the clinical 
      usefulness of the manual RBC count in fish.
CI  - (c) 2014 American Society for Veterinary Clinical Pathology.
FAU - Arnold, Jill E
AU  - Arnold JE
AD  - National Aquarium, Baltimore, MD, USA.
FAU - Matsche, Mark A
AU  - Matsche MA
FAU - Rosemary, Kevin
AU  - Rosemary K
LA  - eng
PT  - Journal Article
DEP - 20141112
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
RN  - 0 (Fixatives)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Animals
MH  - Bass/blood
MH  - Blood Preservation/methods/*veterinary
MH  - Cell Count/*veterinary
MH  - Erythrocyte Count/methods/veterinary
MH  - Fishes/*blood
MH  - *Fixatives
MH  - *Formaldehyde
MH  - Leukocyte Count/methods/veterinary
MH  - Platelet Count/methods/veterinary
MH  - Sharks/blood
OTO - NOTNLM
OT  - Elasmobranch
OT  - hematology
OT  - hemocytometer
OT  - teleost
EDAT- 2014/11/14 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/11/14 06:00
PHST- 2014/11/14 06:00 [entrez]
PHST- 2014/11/14 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1111/vcp.12214 [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2014 Dec;43(4):613-20. doi: 10.1111/vcp.12214. Epub 2014 Nov 12.