PMID- 25393585
OWN - NLM
STAT- MEDLINE
DCOM- 20170427
LR  - 20220408
IS  - 1365-2591 (Electronic)
IS  - 0143-2885 (Linking)
VI  - 48
IP  - 12
DP  - 2015 Dec
TI  - Effects of JNK1/2 on the inflammation cytokine TNF-alpha-enhanced production of MMP-3 
      in human dental pulp fibroblast-like cells.
PG  - 1122-8
LID - 10.1111/iej.12411 [doi]
AB  - AIM: To investigate the effects of the c-Jun N-terminal kinase (JNK1/2) on the 
      inflammation cytokine tumour necrosis factor-alpha (TNF-alpha)-enhanced production of 
      matrix metalloproteinase-3 (MMP-3) in human dental pulp fibroblast-like cells 
      (HPFs). METHODOLOGY: HPFs were grown from pulp explants from healthy donors. 
      Primary cultures were established by culturing the cells for 20 to 30 days. The 
      experiments with HPFs were performed between passages 3 and 10. The HPFs were 
      incubated in serum-free medium containing TNF-alpha for 24 h. The medium in each well 
      was prepared in SDS sample buffer and was analysed for MMP-3 by Western blotting. 
      RESULTS: JNK inhibitor SP601245 markedly inhibited the production of MMP-3 in 
      TNF-alpha-stimulated human dental pulp fibroblasts. MMP-3 production was enhanced by 
      TNF-alpha in HPFs; silencing JNK1 and JNK2 expression inhibited this activation. cAMP 
      response element-binding protein (CREB) was activated by TNF-alpha in HPFs; silencing 
      JNK1 and JNK2 expression inhibited this activation. CONCLUSION: The activation of 
      CREB via JNK pathways in the presence of TNF-alpha occurred with enhancement of MMP-3 
      production in dental pulp fibroblasts.
CI  - (c) 2014 The Authors. International Endodontic Journal published by John Wiley & 
      Sons Ltd on behalf of British Endodontic Society.
FAU - Goda, S
AU  - Goda S
AD  - Department of Biochemistry, Osaka Dental University, Hirakata, Japan.
FAU - Kato, Y
AU  - Kato Y
AD  - Department of Endodontics, Osaka Dental University, Hirakata, Japan.
FAU - Domae, E
AU  - Domae E
AD  - Department of Biochemistry, Osaka Dental University, Hirakata, Japan.
FAU - Hayashi, H
AU  - Hayashi H
AD  - Department of Endodontics, Osaka Dental University, Hirakata, Japan.
FAU - Tani-Ishii, N
AU  - Tani-Ishii N
AD  - Department of Pulp Biology and Endodontics, Kanagawa Dental University, Yokosuka, 
      Japan.
FAU - Iida, J
AU  - Iida J
AD  - Department of Cell Biology, Windber Research Institute, Windber, USA.
FAU - Ikeo, T
AU  - Ikeo T
AD  - Department of Biochemistry, Osaka Dental University, Hirakata, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141224
PL  - England
TA  - Int Endod J
JT  - International endodontic journal
JID - 8004996
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Dental Pulp/*cytology
MH  - Fibroblasts/*metabolism
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors/*physiology
MH  - MAP Kinase Signaling System/drug effects
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Signal Transduction/drug effects
MH  - Tumor Necrosis Factor-alpha/*pharmacology
OTO - NOTNLM
OT  - C-Jun N-terminal kinase
OT  - human dental pulp fibroblast-like cells
OT  - matrix metalloproteinase-3
EDAT- 2014/11/14 06:00
MHDA- 2017/04/28 06:00
CRDT- 2014/11/14 06:00
PHST- 2014/01/24 00:00 [received]
PHST- 2014/11/10 00:00 [accepted]
PHST- 2014/11/14 06:00 [entrez]
PHST- 2014/11/14 06:00 [pubmed]
PHST- 2017/04/28 06:00 [medline]
AID - 10.1111/iej.12411 [doi]
PST - ppublish
SO  - Int Endod J. 2015 Dec;48(12):1122-8. doi: 10.1111/iej.12411. Epub 2014 Dec 24.