PMID- 25394583
OWN - NLM
STAT- MEDLINE
DCOM- 20160203
LR  - 20181113
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 23
IP  - 2
DP  - 2015 Jun
TI  - Chromosome landmarks and autosome-sex chromosome translocations in Rumex 
      hastatulus, a plant with XX/XY1Y2 sex chromosome system.
PG  - 187-97
LID - 10.1007/s10577-014-9446-4 [doi]
AB  - Rumex hastatulus is the North American endemic dioecious plant with heteromorphic 
      sex chromosomes. It is differentiated into two chromosomal races: Texas (T) race 
      characterised by a simple XX/XY sex chromosome system and North Carolina (NC) 
      race with a polymorphic XX/XY1Y2 sex chromosome system. The gross karyotype 
      morphology in NC race resembles the derived type, but chromosomal changes that 
      occurred during its evolution are poorly understood. Our C-banding/DAPI and 
      fluorescence in situ hybridization (FISH) experiments demonstrated that Y 
      chromosomes of both races are enriched in DAPI-positive sequences and that the 
      emergence of polymorphic sex chromosome system was accompanied by the break of 
      ancestral Y chromosome and switch in the localization of 5S rDNA, from autosomes 
      to sex chromosomes (X and Y2). Two contrasting domains were detected within North 
      Carolina Y chromosomes: the older, highly heterochromatinised, inherited from the 
      original Y chromosome and the younger, euchromatic, representing translocated 
      autosomal material. The flow-cytometric DNA estimation showed  approximately 3.5 % genome 
      downsizing in the North Carolina race. Our results are in contradiction to 
      earlier reports on the lack of heterochromatin within Y chromosomes of this 
      species and enable unambiguous identification of autosomes involved in the 
      autosome-heterosome translocation, providing useful chromosome landmarks for 
      further studies on the karyotype and sex chromosome differentiation in this 
      species.
FAU - Grabowska-Joachimiak, Aleksandra
AU  - Grabowska-Joachimiak A
AD  - Department of Plant Breeding and Seed Science, University of Agriculture in 
      Cracow, Lobzowska 24, 31-140, Cracow, Poland, rrjoachi@cyf-kr.edu.pl.
FAU - Kula, Adam
AU  - Kula A
FAU - Ksiazczyk, Tomasz
AU  - Ksiazczyk T
FAU - Chojnicka, Joanna
AU  - Chojnicka J
FAU - Sliwinska, Elwira
AU  - Sliwinska E
FAU - Joachimiak, Andrzej J
AU  - Joachimiak AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141114
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Banding
MH  - *Chromosomes, Plant
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Rumex/*genetics
MH  - *Sex Chromosomes
MH  - *Translocation, Genetic
PMC - PMC4430600
EDAT- 2014/11/15 06:00
MHDA- 2016/02/04 06:00
PMCR- 2014/11/14
CRDT- 2014/11/15 06:00
PHST- 2014/09/03 00:00 [received]
PHST- 2014/10/28 00:00 [accepted]
PHST- 2014/10/26 00:00 [revised]
PHST- 2014/11/15 06:00 [entrez]
PHST- 2014/11/15 06:00 [pubmed]
PHST- 2016/02/04 06:00 [medline]
PHST- 2014/11/14 00:00 [pmc-release]
AID - 9446 [pii]
AID - 10.1007/s10577-014-9446-4 [doi]
PST - ppublish
SO  - Chromosome Res. 2015 Jun;23(2):187-97. doi: 10.1007/s10577-014-9446-4. Epub 2014 
      Nov 14.