PMID- 25394899
OWN - NLM
STAT- MEDLINE
DCOM- 20150615
LR  - 20181113
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 36
IP  - 3
DP  - 2015 Mar
TI  - Methylation of miR124a-1, miR124a-2, and miR124a-3 in Hodgkin lymphoma.
PG  - 1963-71
LID - 10.1007/s13277-014-2802-3 [doi]
AB  - Deregulation of the microRNA miR124a by DNA methylation has been implicated in 
      various malignancies, but no study reported its methylation status in Hodgkin 
      lymphoma (HL). We evaluated the methylation of the three loci encoding for 
      miR124a using methylation-specific PCR in 64 HL patients and 15 reactive lymph 
      nodes obtained from patients with nonmalignant diseases. Results were correlated 
      with clinicopathological parameters. Methylation rates of miR124a-1, miR124a-2, 
      and miR124a-3 in HL were 17, 50, and 28%, respectively. None of the nontumoral 
      samples showed aberrant hypermethylation in any of the miR tested. In HL cases, 
      we found that miR124a-1 methylation correlates with high-risk International 
      Prognostic Score (IPS) (score >3, p = 0.04) and that miR124a-2 methylation was 
      more frequent in children (82.3%, p = 0.006) and men (63.9%, p = 0.01). 
      Methylation of miR124a-3 was associated with advanced Ann-Arbor stages 
      (p = 0.007). The survival analysis showed that methylation of at least one of the 
      miR124a genes was associated with shortened event-free survival in univariate 
      (p = 0.03) and multivariate (p = 0.02) analyses. These results suggest that 
      miR124a methylation is associated with aggressive HL disease and may be an 
      interesting factor for predicting treatment response.
FAU - Ben Dhiab, M
AU  - Ben Dhiab M
AD  - Department of Pathology, Farhat-Hached Hospital of Sousse, Sousse, 4000, Tunisia.
FAU - Ziadi, S
AU  - Ziadi S
FAU - Ksiaa, F
AU  - Ksiaa F
FAU - Louhichi, T
AU  - Louhichi T
FAU - Ben Gacem, R
AU  - Ben Gacem R
FAU - Ben Zineb, A
AU  - Ben Zineb A
FAU - Amara, K
AU  - Amara K
FAU - Hachana, M
AU  - Hachana M
FAU - Trimeche, Mounir
AU  - Trimeche M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141115
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *DNA Methylation
MH  - Disease-Free Survival
MH  - Female
MH  - Hodgkin Disease/diagnosis/*genetics/pathology
MH  - Humans
MH  - Lymph Nodes/pathology
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Prognosis
MH  - Young Adult
EDAT- 2014/11/15 06:00
MHDA- 2015/06/16 06:00
CRDT- 2014/11/15 06:00
PHST- 2014/08/11 00:00 [received]
PHST- 2014/11/03 00:00 [accepted]
PHST- 2014/11/15 06:00 [entrez]
PHST- 2014/11/15 06:00 [pubmed]
PHST- 2015/06/16 06:00 [medline]
AID - 10.1007/s13277-014-2802-3 [doi]
PST - ppublish
SO  - Tumour Biol. 2015 Mar;36(3):1963-71. doi: 10.1007/s13277-014-2802-3. Epub 2014 
      Nov 15.