PMID- 25394900
OWN - NLM
STAT- MEDLINE
DCOM- 20150615
LR  - 20181113
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 36
IP  - 3
DP  - 2015 Mar
TI  - MicroRNA-224 and its target CAMKK2 synergistically influence tumor progression 
      and patient prognosis in prostate cancer.
PG  - 1983-91
LID - 10.1007/s13277-014-2805-0 [doi]
AB  - We previously demonstrated that microRNA (miR)-224 expression was significantly 
      reduced in human prostate cancer (PCa) tissues and predicted unfavorable 
      prognosis in patients. However, the underlying mechanisms of miR-224 have not 
      been fully elucidated. In this study, calcium/calmodulin-dependent protein kinase 
      kinase 2 (CAMKK2) was identified as a target gene of miR-224. Then, we found that 
      enforced expression of miR-224 could suppress PCa cell proliferation and cell 
      cycle by regulating the expression of CAMKK2 in vitro. In addition, the 
      expression levels of miR-224 in PCa tissues were negatively correlated with those 
      of CAMKK2 mRNA significantly (Spearman's correlation: r = -0.66, P = 0.004). 
      Moreover, combined low miR-224 expression and high CAMKK2 expression 
      (miR-224-low/CAMKK2-high) was closely correlated with advanced clinical stage 
      (P = 0.028). Furthermore, PCa patients with miR-224-low/CAMKK2-high expression 
      more frequently had shorter overall survival than those in groups with other 
      expression patterns of two molecules. In conclusion, our data offer the 
      convincing evidence that miR-224 and its target gene CAMKK2 may synergistically 
      contribute to the malignant progression of PCa. Combined detection of miR-224 and 
      CAMKK2 expressions represents an efficient predictor of patient prognosis and may 
      be a novel marker which can provide additional prognostic information in PCa.
FAU - Fu, Hao
AU  - Fu H
AD  - Guangdong Provincial Institute of Nephrology, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - He, Hui-chan
AU  - He HC
FAU - Han, Zhao-dong
AU  - Han ZD
FAU - Wan, Yue-ping
AU  - Wan YP
FAU - Luo, Hong-wei
AU  - Luo HW
FAU - Huang, Ya-qiang
AU  - Huang YQ
FAU - Cai, Chao
AU  - Cai C
FAU - Liang, Yu-xiang
AU  - Liang YX
FAU - Dai, Qi-shan
AU  - Dai QS
FAU - Jiang, Fu-neng
AU  - Jiang FN
FAU - Zhong, Wei-de
AU  - Zhong WD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141115
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MIRN224 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.17 (CAMKK2 protein, human)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Kinase)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Kinase/*genetics/*metabolism
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Disease Progression
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics/*metabolism
MH  - Middle Aged
MH  - Prognosis
MH  - Prostatic Neoplasms/*genetics/*metabolism/pathology
MH  - RNA, Messenger/genetics
EDAT- 2014/11/15 06:00
MHDA- 2015/06/16 06:00
CRDT- 2014/11/15 06:00
PHST- 2014/08/04 00:00 [received]
PHST- 2014/11/03 00:00 [accepted]
PHST- 2014/11/15 06:00 [entrez]
PHST- 2014/11/15 06:00 [pubmed]
PHST- 2015/06/16 06:00 [medline]
AID - 10.1007/s13277-014-2805-0 [doi]
PST - ppublish
SO  - Tumour Biol. 2015 Mar;36(3):1983-91. doi: 10.1007/s13277-014-2805-0. Epub 2014 
      Nov 15.