PMID- 25395292
OWN - NLM
STAT- MEDLINE
DCOM- 20150910
LR  - 20181202
IS  - 2211-0690 (Electronic)
IS  - 2211-0682 (Linking)
VI  - 20
IP  - 1
DP  - 2015 Feb
TI  - Reagent and labor cost optimization through automation of fluorescence in situ 
      hybridization (FISH) with the VP 2000: an Italian case study.
PG  - 25-31
LID - 10.1177/2211068214558294 [doi]
AB  - In the modern molecular diagnostic laboratory, cost considerations are of 
      paramount importance. Automation of complex molecular assays not only allows a 
      laboratory to accommodate higher test volumes and throughput but also has a 
      considerable impact on the cost of testing from the perspective of reagent costs, 
      as well as hands-on time for skilled laboratory personnel. The following study 
      tracked the cost of labor (hands-on time) and reagents for fluorescence in situ 
      hybridization (FISH) testing in a routine, high-volume pathology and cytogenetics 
      laboratory in Treviso, Italy, over a 2-y period (2011-2013). The laboratory 
      automated FISH testing with the VP 2000 Processor, a deparaffinization, 
      pretreatment, and special staining instrument produced by Abbott Molecular, and 
      compared hands-on time and reagent costs to manual FISH testing. The results 
      indicated significant cost and time saving when automating FISH with VP 2000 when 
      more than six FISH tests were run per week. At 12 FISH assays per week, an 
      approximate total cost reduction of 55% was observed. When running 46 FISH 
      specimens per week, the cost saving increased to 89% versus manual testing. The 
      results demonstrate that the VP 2000 processor can significantly reduce the cost 
      of FISH testing in diagnostic laboratories.
CI  - (c) 2014 Society for Laboratory Automation and Screening.
FAU - Zanatta, Lucia
AU  - Zanatta L
AD  - Department of Oncology and Pathology, General Hospital of Treviso, Treviso, Italy 
      luzanatta@ulss.tv.it.
FAU - Valori, Laura
AU  - Valori L
AD  - Department of Oncology and Pathology, General Hospital of Treviso, Treviso, 
      Italy.
FAU - Cappelletto, Eleonora
AU  - Cappelletto E
AD  - Department of Oncology and Pathology, General Hospital of Treviso, Treviso, 
      Italy.
FAU - Pozzebon, Maria Elena
AU  - Pozzebon ME
AD  - Department of Oncology and Pathology, General Hospital of Treviso, Treviso, 
      Italy.
FAU - Pavan, Elisabetta
AU  - Pavan E
AD  - Department of Oncology and Pathology, General Hospital of Treviso, Treviso, 
      Italy.
FAU - Dei Tos, Angelo Paolo
AU  - Dei Tos AP
AD  - Department of Oncology and Pathology, General Hospital of Treviso, Treviso, 
      Italy.
FAU - Merkle, Dennis
AU  - Merkle D
AD  - Abbott Molecular, Max-Planck-Ring 2, Wiesbaden, Germany.
LA  - eng
PT  - Journal Article
DEP - 20141113
PL  - United States
TA  - J Lab Autom
JT  - Journal of laboratory automation
JID - 101558509
RN  - 0 (Indicators and Reagents)
SB  - IM
EIN - J Lab Autom. 2016 Feb;21(1):222. PMID: 26792900
MH  - Automation, Laboratory/*economics/*methods
MH  - Cytogenetics/economics/methods
MH  - Health Workforce/*economics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*economics/*methods
MH  - Indicators and Reagents/*economics
MH  - Italy
MH  - Pathology/economics/methods
MH  - Time Factors
OTO - NOTNLM
OT  - FISH
OT  - VP 2000
OT  - automation
OT  - cost reduction
OT  - fluorescence in situ hybridization
EDAT- 2014/11/15 06:00
MHDA- 2015/09/12 06:00
CRDT- 2014/11/15 06:00
PHST- 2014/11/15 06:00 [entrez]
PHST- 2014/11/15 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
AID - 2211068214558294 [pii]
AID - 10.1177/2211068214558294 [doi]
PST - ppublish
SO  - J Lab Autom. 2015 Feb;20(1):25-31. doi: 10.1177/2211068214558294. Epub 2014 Nov 
      13.