PMID- 25398007
OWN - NLM
STAT- MEDLINE
DCOM- 20151230
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 11
DP  - 2014
TI  - Regional difference in sex steroid action on formation of morphological sex 
      differences in the anteroventral periventricular nucleus and principal nucleus of 
      the bed nucleus of the stria terminalis.
PG  - e112616
LID - 10.1371/journal.pone.0112616 [doi]
LID - e112616
AB  - Sex steroid action is critical to form sexually dimorphic nuclei, although it is 
      not fully understood. We previously reported that masculinization of the 
      principal nucleus of the bed nucleus of the stria terminalis (BNSTp), which is 
      larger and has more neurons in males than in females, involves aromatized 
      testosterone that acts via estrogen receptor-alpha (ERalpha), but not estrogen receptor-beta 
      (ERbeta). Here, we examined sex steroid action on the formation of the anteroventral 
      periventricular nucleus (AVPV) that is larger and has more neurons in females. 
      Morphometrical analysis of transgenic mice lacking aromatase, ERalpha, or ERbeta genes 
      revealed that the volume and neuron number of the male AVPV were significantly 
      increased by deletion of aromatase and ERalpha genes, but not the ERbeta gene. We 
      further examined the AVPV and BNSTp of androgen receptor knockout (ARKO) mice. 
      The volume and neuron number of the male BNSTp were smaller in ARKO mice than 
      those in wild-type mice, while no significant effect of ARKO was found on the 
      AVPV and female BNSTp. We also examined aromatase, ERalpha, and AR mRNA levels in the 
      AVPV and BNSTp of wild-type and ARKO mice on embryonic day (ED) 18 and postnatal 
      day (PD) 4. AR mRNA in the BNSTp and AVPV of wild-type mice was not expressed on 
      ED18 and emerged on PD4. In the AVPV, the aromatase mRNA level was higher on 
      ED18, although the ERalpha mRNA level was higher on PD4 without any effect of AR gene 
      deletion. Aromatase and ERalpha mRNA levels in the male BNSTp were significantly 
      increased on PD4 by AR gene deletion. These results suggest that estradiol 
      signaling via ERalpha during the perinatal period and testosterone signaling via AR 
      during the postnatal period are required for masculinization of the BNSTp, 
      whereas the former is sufficient to defeminize the AVPV.
FAU - Kanaya, Moeko
AU  - Kanaya M
AD  - Division of Life Science, Graduate School of Science and Engineering, Saitama 
      University, Saitama, Japan.
FAU - Tsuda, Mumeko C
AU  - Tsuda MC
AD  - Laboratory of Behavioral Neuroendocrinology, University of Tsukuba, Tsukuba, 
      Japan.
FAU - Sagoshi, Shoko
AU  - Sagoshi S
AD  - Laboratory of Behavioral Neuroendocrinology, University of Tsukuba, Tsukuba, 
      Japan.
FAU - Nagata, Kazuyo
AU  - Nagata K
AD  - Laboratory of Behavioral Neuroendocrinology, University of Tsukuba, Tsukuba, 
      Japan.
FAU - Morimoto, Chihiro
AU  - Morimoto C
AD  - Laboratory of Behavioral Neuroendocrinology, University of Tsukuba, Tsukuba, 
      Japan.
FAU - Thu, Chaw Kyi Tha
AU  - Thu CK
AD  - Division of Life Science, Graduate School of Science and Engineering, Saitama 
      University, Saitama, Japan.
FAU - Toda, Katsumi
AU  - Toda K
AD  - Department of Biochemistry, School of Medicine, Kochi University, Nankoku, Japan.
FAU - Kato, Shigeaki
AU  - Kato S
AD  - Soma Central Hospital, Fukushima, Japan.
FAU - Ogawa, Sonoko
AU  - Ogawa S
AD  - Laboratory of Behavioral Neuroendocrinology, University of Tsukuba, Tsukuba, 
      Japan.
FAU - Tsukahara, Shinji
AU  - Tsukahara S
AD  - Division of Life Science, Graduate School of Science and Engineering, Saitama 
      University, Saitama, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141114
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA Primers)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Receptors, Androgen)
RN  - 3XMK78S47O (Testosterone)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Aromatase/metabolism
MH  - Body Weights and Measures
MH  - DNA Primers/genetics
MH  - Estrogen Receptor alpha/*metabolism
MH  - Female
MH  - Gene Expression Profiling
MH  - Hypothalamus, Anterior/*embryology/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Receptors, Androgen/genetics
MH  - Septal Nuclei/*embryology/*metabolism
MH  - *Sex Characteristics
MH  - Testosterone/*metabolism
PMC - PMC4232352
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/11/15 06:00
MHDA- 2015/12/31 06:00
PMCR- 2014/11/14
CRDT- 2014/11/15 06:00
PHST- 2014/06/10 00:00 [received]
PHST- 2014/10/02 00:00 [accepted]
PHST- 2014/11/15 06:00 [entrez]
PHST- 2014/11/15 06:00 [pubmed]
PHST- 2015/12/31 06:00 [medline]
PHST- 2014/11/14 00:00 [pmc-release]
AID - PONE-D-14-25651 [pii]
AID - 10.1371/journal.pone.0112616 [doi]
PST - epublish
SO  - PLoS One. 2014 Nov 14;9(11):e112616. doi: 10.1371/journal.pone.0112616. 
      eCollection 2014.