PMID- 25399100
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20141117
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1247
DP  - 2015
TI  - Fluorescence in situ hybridization for the tissue detection of bacterial 
      pathogens associated with porcine infections.
PG  - 219-34
LID - 10.1007/978-1-4939-2004-4_17 [doi]
AB  - Fluorescence in situ hybridization (FISH) is an efficient technique for the 
      identification of specific bacteria in tissue of both experimental and 
      spontaneous infections. The method detects specific sequences of nucleic acids by 
      hybridization of fluorescently labeled probes to complementary target sequences 
      within intact cells. FISH allows direct histological localization of the bacteria 
      in the tissue and thereby a correlation between the infection and the 
      histopathological changes present. This chapter presents protocols for FISH 
      identification of bacterial pathogens in fixed deparaffinized tissue samples 
      mounted on glass slides. Two different methods are presented: one is illustrated 
      with the use of peptide nucleic acid (PNA) that is carried out directly on glass 
      slides (Method I), whereas the other is exemplified by using a DNA probe in a 
      Shandon rack (Method II). In the two methods, both PNA and DNA probes can be 
      used.
FAU - Jensen, Henrik Elvang
AU  - Jensen HE
AD  - Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Ridebanevej 3, 1870, Frederiksberg C, Denmark, 
      elvang@life.ku.dk.
FAU - Jensen, Louise Kruse
AU  - Jensen LK
FAU - Barington, Kristiane
AU  - Barington K
FAU - Pors, Susanne Elisabeth
AU  - Pors SE
FAU - Bjarnsholt, Thomas
AU  - Bjarnsholt T
FAU - Boye, Mette
AU  - Boye M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Nucleic Acid Probes)
SB  - IM
MH  - Animals
MH  - Bacteria/*genetics
MH  - Bacterial Infections/*veterinary
MH  - Histocytological Preparation Techniques
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Probes
MH  - Swine
MH  - Swine Diseases/*diagnosis/*microbiology
EDAT- 2014/11/17 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/11/17 06:00
PHST- 2014/11/17 06:00 [entrez]
PHST- 2014/11/17 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1007/978-1-4939-2004-4_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1247:219-34. doi: 10.1007/978-1-4939-2004-4_17.