PMID- 25399101
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20141117
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1247
DP  - 2015
TI  - Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry.
PG  - 235-43
LID - 10.1007/978-1-4939-2004-4_18 [doi]
AB  - Species of the family Pasteurellaceae play an important role as primary or 
      opportunistic, predominantly respiratory, pathogens in domestic and wild animals. 
      Some of them cause severe disease with high economic losses in commercial animal 
      husbandry. Hence, rapid and accurate differentiation of Pasteurellaceae is 
      important and signifies a particular challenge to diagnostic laboratories. 
      Identification and differentiation of Pasteurellaceae is mostly done using 
      phenotypic tests or genetic identification based on sequence similarity of 
      housekeeping genes, such as the rrs gene encoding the 16S ribosomal RNA (16S 
      rRNA). Both approaches are time consuming, laborious, and costly, therefore often 
      delaying the final diagnosis of disease or epidemics. Matrix-assisted laser 
      desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry represents an 
      alternative rapid and reliable method for the differentiation of most members of 
      the family Pasteurellaceae. It is able to differentiate within a few minutes the 
      currently known 18 genera and most of the over 60 species and subspecies of 
      Pasteurellaceae including many members encountered in veterinary diagnostic 
      laboratories. A few closely related species and subspecies that cannot be 
      discriminated by MALDI-TOF are easily identified further by complementary simple 
      tests, such as hemolysis done simultaneously or routinely during pathogen 
      isolation.
FAU - Frey, Joachim
AU  - Frey J
AD  - Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, 
      Bern, Switzerland, joachim.frey@vetsuisse.unibe.ch.
FAU - Kuhnert, Peter
AU  - Kuhnert P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animal Diseases/*diagnosis/*microbiology
MH  - Animals
MH  - Databases, Factual
MH  - *Pasteurellaceae
MH  - Pasteurellaceae Infections/*veterinary
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 2014/11/17 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/11/17 06:00
PHST- 2014/11/17 06:00 [entrez]
PHST- 2014/11/17 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1007/978-1-4939-2004-4_18 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1247:235-43. doi: 10.1007/978-1-4939-2004-4_18.