PMID- 25400454 OWN - NLM STAT- MEDLINE DCOM- 20150817 LR - 20191224 IS - 2219-2840 (Electronic) IS - 1007-9327 (Print) IS - 1007-9327 (Linking) VI - 20 IP - 42 DP - 2014 Nov 14 TI - Chunggan extract, a traditional herbal formula, ameliorated alcohol-induced hepatic injury in rat model. PG - 15703-14 LID - 10.3748/wjg.v20.i42.15703 [doi] AB - AIM: To evaluate protective effects of Chunggan extract (CGX), a traditional herbal formula, under 4 wk of alcohol consumption-induced liver injury. METHODS: Male Sprague-Dawley Rats were orally administered 30% ethanol daily for 4 wk with or without CGX. The pharmaceutical properties were assessed through liver enzymes, histopathology, fibrogenic cytokines, and alcohol metabolism in hepatic tissues as well as by in vitro experiment using HSC-T6 cells. RESULTS: Four weeks of alcohol consumption notably increased liver enzymes and malondialdehyde levels in serum and hepatic tissue. CGX not only prevented the collagen deposition determined by histopathology and hydroxyproline content, but also normalized transforming growth factor-beta, platelet-derived growth factor-beta and connective tissue growth factor at the gene expression and protein levels in liver tissue. Moreover, CGX treatment also significantly normalized the abnormal changes in gene expression profiles of extracellular matrix proteins, matrix metalloproteinase and their inhibitors, alcohol metabolism, and inflammatory reactions. In the acetaldehyde-stimulated HSC-T6 cells, CGX considerably inhibited collagen production and normalized fibrogenic cytokines in both gene expression and protein levels. CONCLUSION: The present study evidenced that CGX has hepatoprotective properties via modulation of fibrogenic cytokines and alcohol metabolism in alcoholic liver injury. FAU - Kim, Hyeong-Geug AU - Kim HG AD - Hyeong-Geug Kim, Jong-Min Han, Jin-Seok Lee, Min-Kyung Choi, Yeon-Hwa Park, Chang-Gue Son, Liver and immunology research center, Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University, Daejeon 301-724, South Korea. FAU - Kim, Jung-Min AU - Kim JM AD - Hyeong-Geug Kim, Jong-Min Han, Jin-Seok Lee, Min-Kyung Choi, Yeon-Hwa Park, Chang-Gue Son, Liver and immunology research center, Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University, Daejeon 301-724, South Korea. FAU - Han, Jong-Min AU - Han JM AD - Hyeong-Geug Kim, Jong-Min Han, Jin-Seok Lee, Min-Kyung Choi, Yeon-Hwa Park, Chang-Gue Son, Liver and immunology research center, Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University, Daejeon 301-724, South Korea. FAU - Lee, Jin-Seok AU - Lee JS AD - Hyeong-Geug Kim, Jong-Min Han, Jin-Seok Lee, Min-Kyung Choi, Yeon-Hwa Park, Chang-Gue Son, Liver and immunology research center, Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University, Daejeon 301-724, South Korea. FAU - Choi, Min-Kyung AU - Choi MK AD - Hyeong-Geug Kim, Jong-Min Han, Jin-Seok Lee, Min-Kyung Choi, Yeon-Hwa Park, Chang-Gue Son, Liver and immunology research center, Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University, Daejeon 301-724, South Korea. FAU - Lee, Dong-Soo AU - Lee DS AD - Hyeong-Geug Kim, Jong-Min Han, Jin-Seok Lee, Min-Kyung Choi, Yeon-Hwa Park, Chang-Gue Son, Liver and immunology research center, Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University, Daejeon 301-724, South Korea. FAU - Park, Yeon-Hwa AU - Park YH AD - Hyeong-Geug Kim, Jong-Min Han, Jin-Seok Lee, Min-Kyung Choi, Yeon-Hwa Park, Chang-Gue Son, Liver and immunology research center, Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University, Daejeon 301-724, South Korea. FAU - Son, Chang-Gue AU - Son CG AD - Hyeong-Geug Kim, Jong-Min Han, Jin-Seok Lee, Min-Kyung Choi, Yeon-Hwa Park, Chang-Gue Son, Liver and immunology research center, Daejeon Oriental Hospital of Oriental Medical Collage of Daejeon University, Daejeon 301-724, South Korea. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - World J Gastroenterol JT - World journal of gastroenterology JID - 100883448 RN - 0 (CCN2 protein, rat) RN - 0 (Cytokines) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Protective Agents) RN - 0 (Proto-Oncogene Proteins c-sis) RN - 0 (RNA, Messenger) RN - 0 (Transforming Growth Factor beta) RN - 0 (chunggan) RN - 139568-91-5 (Connective Tissue Growth Factor) RN - 4Y8F71G49Q (Malondialdehyde) RN - 9007-34-5 (Collagen) RN - RMB44WO89X (Hydroxyproline) SB - IM MH - Animals MH - Cell Line MH - Collagen/metabolism MH - Connective Tissue Growth Factor/genetics/metabolism MH - Cytokines/genetics/metabolism MH - Disease Models, Animal MH - Drugs, Chinese Herbal/*pharmacology MH - Gene Expression Regulation MH - Hepatocytes/*drug effects/metabolism/pathology MH - Hydroxyproline/metabolism MH - Liver/*drug effects/metabolism/pathology MH - Liver Diseases, Alcoholic/blood/genetics/pathology/*prevention & control MH - Male MH - Malondialdehyde/blood MH - Phytotherapy MH - Plants, Medicinal MH - Protective Agents/*pharmacology MH - Proto-Oncogene Proteins c-sis/genetics/metabolism MH - RNA, Messenger/metabolism MH - Rats, Sprague-Dawley MH - Time Factors MH - Transforming Growth Factor beta/genetics/metabolism PMC - PMC4229535 OTO - NOTNLM OT - Alcohol abuse OT - Fibrogenic cytokines OT - Hepato stellate cell OT - Liver injury OT - Traditional Chinese Medicine EDAT- 2014/11/18 06:00 MHDA- 2015/08/19 06:00 PMCR- 2014/11/14 CRDT- 2014/11/18 06:00 PHST- 2014/04/02 00:00 [received] PHST- 2014/05/14 00:00 [revised] PHST- 2014/06/21 00:00 [accepted] PHST- 2014/11/18 06:00 [entrez] PHST- 2014/11/18 06:00 [pubmed] PHST- 2015/08/19 06:00 [medline] PHST- 2014/11/14 00:00 [pmc-release] AID - 10.3748/wjg.v20.i42.15703 [doi] PST - ppublish SO - World J Gastroenterol. 2014 Nov 14;20(42):15703-14. doi: 10.3748/wjg.v20.i42.15703.