PMID- 25400755
OWN - NLM
STAT- MEDLINE
DCOM- 20150709
LR  - 20220317
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 7
IP  - 10
DP  - 2014
TI  - Quantitative RT-PCR assay of HER2 mRNA expression in formalin-fixed and 
      paraffin-embedded breast cancer tissues.
PG  - 6752-9
AB  - Detection of human epidermal growth factor receptor 2 gene (HER2, also known as 
      erbB2) expression is a preparatory process to decide a treatment strategy for 
      breast cancer patients. 20-30% of breast cancer patients have HER2 
      overexpression, and they usually show poor recovery rate. For detection of HER2 
      expression, immunohistochemistry (IHC) and fluorescence in situ hybridization 
      (FISH) methods are conventionally used. Although these methods are accurate and 
      reliable, their time-consuming process and high cost need a concise method with 
      high sensitivity and accuracy. As a complementary method to the current IHC/FISH 
      standard techniques, PCR-based methods have been developed. Here we employed a 
      quantitative PCR method to detect HER2 expression in one hundred ninety nine 
      formalin-fixed and paraffin-embedded (FFPE) breast cancer tissue samples from the 
      patients treated over two years at the Yonsei University Severance Hospital, 
      Republic of Korea. Relative expression of HER2 mRNA in the FFPE samples was 
      analyzed using a quantitative RT-PCR (RT-qPCR) method and the obtained HER2 
      expression levels were compared with those from IHC/FISH methods. Our results 
      show that the RT-qPCR method was highly concordant with IHC/FISH methods for 
      detecting HER2 expression. Overall sensitivity and specificity of the BrightGen 
      HER2 RT-qDx assay kit (Syantra, Calgary, Canada), which is a kit we used for 
      RT-qPCR analyses, were 93.0% and 89.8% (P < 0.0001), respectively. The diagnostic 
      cut-off value of HER2 RT-qDx for the clinical samples was determined by 
      likelihood ratio, among which the highest likelihood ratio of relative HER2 mRNA 
      levels was over 105.5 (AUC = 0.9466) with the highest sensitivity and 
      specificity. Our study indicates that quantification of HER2 mRNA expression with 
      the RT-qPCR could be an alternative method of conventional IHC/FISH methods.
FAU - Park, Sangjung
AU  - Park S
AD  - Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei 
      University Gangwon, Republic of Korea ; Department of Clinical Laboratory 
      Science, College of Medical Science, Daegu Haany University Daegu, Republic of 
      Korea.
FAU - Wang, Hye-Young
AU  - Wang HY
AD  - M&D, Inc., Wonju Eco Environmental Technology Center Gangwon, Republic of Korea.
FAU - Kim, Sunghyun
AU  - Kim S
AD  - Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei 
      University Gangwon, Republic of Korea ; Institute for Life Science and 
      Biotechnology, Yonsei University Seoul, Republic of Korea.
FAU - Ahn, Sungwoo
AU  - Ahn S
AD  - Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei 
      University Gangwon, Republic of Korea.
FAU - Lee, Dongsup
AU  - Lee D
AD  - Department of Clinical Laboratory Science, Hyejeon College Chungnam, Republic of 
      Korea.
FAU - Cho, Yoonjung
AU  - Cho Y
AD  - Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei 
      University Gangwon, Republic of Korea.
FAU - Park, Kwang Hwa
AU  - Park KH
AD  - Department of Pathology, Wonju College of Medicine, Yonsei University Gangwon, 
      Republic of Korea.
FAU - Jung, Dongju
AU  - Jung D
AD  - Department of Biomedical Laboratory Science, College of Natural Sciences, Hoseo 
      University Chungnam, Republic of Korea.
FAU - Kim, Seung Il
AU  - Kim SI
AD  - Department of Surgery, Yonsei University College of Medicine Seoul, Republic of 
      Korea.
FAU - Lee, Hyeyoung
AU  - Lee H
AD  - Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei 
      University Gangwon, Republic of Korea.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140915
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Fixatives)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Biopsy
MH  - Breast Neoplasms/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Female
MH  - *Fixatives
MH  - *Formaldehyde
MH  - Hospitals, University
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - *Paraffin Embedding
MH  - Predictive Value of Tests
MH  - RNA, Messenger/*analysis
MH  - Reagent Kits, Diagnostic
MH  - *Real-Time Polymerase Chain Reaction/instrumentation
MH  - Receptor, ErbB-2/*genetics
MH  - Republic of Korea
MH  - *Reverse Transcriptase Polymerase Chain Reaction/instrumentation
MH  - Tissue Fixation/*methods
PMC - PMC4230085
OTO - NOTNLM
OT  - Breast cancer
OT  - FFPE
OT  - HER2
OT  - RT-qPCR
OT  - molecular diagnosis
EDAT- 2014/11/18 06:00
MHDA- 2015/07/15 06:00
PMCR- 2014/09/15
CRDT- 2014/11/18 06:00
PHST- 2014/06/16 00:00 [received]
PHST- 2014/07/29 00:00 [accepted]
PHST- 2014/11/18 06:00 [entrez]
PHST- 2014/11/18 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
PHST- 2014/09/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2014 Sep 15;7(10):6752-9. eCollection 2014.