PMID- 25401495
OWN - NLM
STAT- MEDLINE
DCOM- 20160119
LR  - 20240314
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 11
DP  - 2014
TI  - Characterization of the secretomes of two vibrios pathogenic to mollusks.
PG  - e113097
LID - 10.1371/journal.pone.0113097 [doi]
LID - e113097
AB  - Vibrio tapetis causes the brown ring disease in the Japanese clam Ruditapes 
      philippinarum while Vibrio aestuarianus is associated with massive oyster 
      mortalities. As extracellular proteins are often associated with the virulence of 
      pathogenic bacteria, we undertook a proteomic approach to characterize the 
      secretomes of both vibrios. The extracellular proteins (ECPs) of both species 
      were fractionated by SEC-FPLC and in vitro assays were performed to measure the 
      effects of each fraction on hemocyte cellular parameters (phagocytosis and 
      adhesion). Fractions showing a significant effect were subjected to SDS-PAGE, and 
      proteins were identified by nano LC-MS/MS. 45 proteins were identified for V. 
      aestuarianus and 87 for V. tapetis. Most of them belonged to outer membrane or 
      were periplasmic, including porins or adhesins that were already described as 
      virulence factors in other bacterial species. Others were transporter components, 
      flagella proteins, or proteins of unknown function (14 and 15 respectively). 
      Interestingly, for V. aestuarianus, we noted the secretion of 3 extracellular 
      enzymes including the Vam metalloprotease and two other enzymes (one putative 
      lipase and one protease). For V. tapetis, we identified five extracellular 
      enymes, i.e. two different endochitinases, one protease, one lipase and an 
      adhesin. A comparison of both secretomes also showed that only the putative 
      extracellular lipase was common to both secretomes, underscoring the difference 
      in pathogenicity mechanisms between these two species. Overall, these results 
      characterize for the first time the secretomes of these two marine pathogenic 
      vibrios and constitute a useful working basis to further analyze the contribution 
      of specific proteins in the virulence mechanisms of these species.
FAU - Madec, Stephanie
AU  - Madec S
AD  - Laboratoire Universitaire de Biodiversite et Ecologie Microbienne (EA3882), SFR48 
      ScInBios, Universite de Bretagne Occidentale (UBO), UEB, ESIAB, Technopole Brest 
      Iroise, 29280, Plouzane, France.
FAU - Pichereau, Vianney
AU  - Pichereau V
AD  - Laboratoire des Sciences de l'Environnement Marin, UMR 6539 UBO/CNRS/IRD/Ifremer, 
      Universite de Bretagne Occidentale (UBO), Institut Universitaire Europeen de la 
      Mer, Technopole Brest Iroise, 29280, Plouzane, France.
FAU - Jacq, Annick
AU  - Jacq A
AD  - Institut de Genetique et de Microbiologie, UMR8621, CNRS-Universite Paris-Sud, 
      91405, Orsay, France.
FAU - Paillard, Mathieu
AU  - Paillard M
AD  - Laboratoire des Sciences de l'Environnement Marin, UMR 6539 UBO/CNRS/IRD/Ifremer, 
      Universite de Bretagne Occidentale (UBO), Institut Universitaire Europeen de la 
      Mer, Technopole Brest Iroise, 29280, Plouzane, France.
FAU - Boisset, Claire
AU  - Boisset C
AD  - Centre de Recherche sur les macromolecules vegetales, CERMAV-CNRS, BP53, 38041 
      Grenoble, France.
FAU - Guerard, Fabienne
AU  - Guerard F
AD  - Laboratoire des Sciences de l'Environnement Marin, UMR 6539 UBO/CNRS/IRD/Ifremer, 
      Universite de Bretagne Occidentale (UBO), Institut Universitaire Europeen de la 
      Mer, Technopole Brest Iroise, 29280, Plouzane, France.
FAU - Paillard, Christine
AU  - Paillard C
AD  - Laboratoire des Sciences de l'Environnement Marin, UMR 6539 UBO/CNRS/IRD/Ifremer, 
      Universite de Bretagne Occidentale (UBO), Institut Universitaire Europeen de la 
      Mer, Technopole Brest Iroise, 29280, Plouzane, France.
FAU - Nicolas, Jean-Louis
AU  - Nicolas JL
AD  - Laboratoire des Sciences de l'Environnement Marin, UMR 6539 UBO/CNRS/IRD/Ifremer, 
      Universite de Bretagne Occidentale (UBO), Institut Universitaire Europeen de la 
      Mer, Technopole Brest Iroise, 29280, Plouzane, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141117
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Bacterial Proteins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Hemocytes/metabolism/microbiology
MH  - Mollusca/immunology/*metabolism/*microbiology
MH  - Phagocytosis
MH  - Proteomics/*methods
MH  - Tandem Mass Spectrometry
MH  - Vibrio/classification/*pathogenicity
MH  - Vibrio Infections/immunology/*metabolism/microbiology
MH  - Virulence Factors/*metabolism
PMC - PMC4234667
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/11/18 06:00
MHDA- 2016/01/20 06:00
PMCR- 2014/11/17
CRDT- 2014/11/18 06:00
PHST- 2014/07/21 00:00 [received]
PHST- 2014/10/19 00:00 [accepted]
PHST- 2014/11/18 06:00 [entrez]
PHST- 2014/11/18 06:00 [pubmed]
PHST- 2016/01/20 06:00 [medline]
PHST- 2014/11/17 00:00 [pmc-release]
AID - PONE-D-14-32194 [pii]
AID - 10.1371/journal.pone.0113097 [doi]
PST - epublish
SO  - PLoS One. 2014 Nov 17;9(11):e113097. doi: 10.1371/journal.pone.0113097. 
      eCollection 2014.