PMID- 25402728
OWN - NLM
STAT- MEDLINE
DCOM- 20150814
LR  - 20190221
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 6
IP  - 1
DP  - 2015 Jan 1
TI  - Dendritic cells respond to nasopharygeal carcinoma cells through annexin 
      A2-recognizing DC-SIGN.
PG  - 159-70
AB  - Dendritic cells (DCs) play an essential role in immunity and are used in cancer 
      immunotherapy. However, these cells can be tuned by tumors with immunosuppressive 
      responses. DC-specific intercellular adhesion molecule 3-Grabbing Nonintegrin 
      (DC-SIGN), a C-type lectin expressed on DCs, recognizes certain carbohydrate 
      structures which can be found on cancer cells. Nasopharyngeal carcinoma (NPC) is 
      an epithelial cell-derived malignant tumor, in which immune response remains 
      unclear. This research is to reveal the molecular link on NPC cells that induces 
      the immunosuppressive responses in DCs. In this article, we report identification 
      of annexin A2 (ANXA2) on NPC cells as a ligand for DC-SIGN on DCs. N-linked 
      mannose-rich glycan on ANXA2 may mediate the interaction. ANXA2 was abundantly 
      expressed in NPC, and knockdown of ANXA2 suppressed NPC xenograft in mice, 
      suggesting a crucial role of ANXA2 in NPC growth. Interaction with NPC cells 
      caused DC-SIGN activation in DCs. Consequently DC maturation and the 
      proinflammatory interleukin (IL)-12 production were inhibited, and the 
      immunosuppressive IL-10 production was promoted. Blockage of either DC-SIGN or 
      ANXA2 eliminated the production of IL-10 from DCs. This report suggests that 
      suppression of ANXA2 at its expression or glycosylation on NPC may improve 
      DC-mediated immunotherapy for the tumor.
FAU - Chao, Pin-Zhir
AU  - Chao PZ
AD  - Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan. Department of Otolaryngology, Shuang-Ho Hospital, New 
      Taipei, Taiwan.
FAU - Hsieh, Ming-Shium
AU  - Hsieh MS
AD  - Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan. Department of Orthopedics, En Chu Kong Hospital, New 
      Taipei, Taiwan.
FAU - Cheng, Chao-Wen
AU  - Cheng CW
AD  - Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan.
FAU - Hsu, Tin-Jui
AU  - Hsu TJ
AD  - School of Medical Laboratory Science and Biotechnology, College of Medical 
      Science and Technology, Taipei Medical University, Taipei, Taiwan.
FAU - Lin, Yun-Tien
AU  - Lin YT
AD  - School of Medical Laboratory Science and Biotechnology, College of Medical 
      Science and Technology, Taipei Medical University, Taipei, Taiwan.
FAU - Lai, Chang-Hao
AU  - Lai CH
AD  - School of Medical Laboratory Science and Biotechnology, College of Medical 
      Science and Technology, Taipei Medical University, Taipei, Taiwan.
FAU - Liao, Chen-Chung
AU  - Liao CC
AD  - Proteomics Research Center, National Yang-Ming University, Taipei, Taiwan.
FAU - Chen, Wei-Yu
AU  - Chen WY
AD  - Department of Pathology, Wan Fang Hospital, Taipei, Taiwan.
FAU - Leung, Ting-Kai
AU  - Leung TK
AD  - Department of Radiology, School of Medicine, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan.
FAU - Lee, Fei-Peng
AU  - Lee FP
AD  - Department of Otolaryngology, Head and Neck Surgery, Wan-Fang Medical Center, 
      Taipei, Taiwan.
FAU - Lin, Yung-Feng
AU  - Lin YF
AD  - School of Medical Laboratory Science and Biotechnology, College of Medical 
      Science and Technology, Taipei Medical University, Taipei, Taiwan.
FAU - Chen, Chien-Ho
AU  - Chen CH
AD  - School of Medical Laboratory Science and Biotechnology, College of Medical 
      Science and Technology, Taipei Medical University, Taipei, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (ANXA2 protein, human)
RN  - 0 (Annexin A2)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Ligands)
RN  - 0 (Polysaccharides)
RN  - 0 (Receptors, Cell Surface)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Annexin A2/*metabolism
MH  - Carcinoma
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cell Line, Tumor
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*cytology
MH  - *Gene Expression Regulation, Neoplastic
MH  - Glycosylation
MH  - Humans
MH  - Immunoglobulin G/chemistry
MH  - Immunoprecipitation
MH  - Immunosuppressive Agents/chemistry
MH  - Immunotherapy/methods
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Lectins, C-Type/*metabolism
MH  - Ligands
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Nasopharyngeal Carcinoma
MH  - Nasopharyngeal Neoplasms/*metabolism
MH  - Neoplasm Transplantation
MH  - Polysaccharides/chemistry
MH  - RNA Interference
MH  - Receptors, Cell Surface/*metabolism
PMC - PMC4381585
COIS- Conflict of Interest Disclosures All the authors declare no conflict of interest 
      on the publication.
EDAT- 2014/11/18 06:00
MHDA- 2015/08/15 06:00
PMCR- 2015/01/01
CRDT- 2014/11/18 06:00
PHST- 2014/09/03 00:00 [received]
PHST- 2014/11/06 00:00 [accepted]
PHST- 2014/11/18 06:00 [entrez]
PHST- 2014/11/18 06:00 [pubmed]
PHST- 2015/08/15 06:00 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 2700 [pii]
AID - 10.18632/oncotarget.2700 [doi]
PST - ppublish
SO  - Oncotarget. 2015 Jan 1;6(1):159-70. doi: 10.18632/oncotarget.2700.