PMID- 25404650
OWN - NLM
STAT- MEDLINE
DCOM- 20150629
LR  - 20181113
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 20
DP  - 2014 Nov 14
TI  - Association between reversal in the expression of hyperpolarization-activated 
      cyclic nucleotide-gated (HCN) channel and age-related atrial fibrillation.
PG  - 2292-7
LID - 10.12659/MSM.892505 [doi]
AB  - BACKGROUND: We compared cardiac electrophysiological indicators and regional 
      expression levels of cardiac hyperpolarization-activated cyclic nucleotide-gated 
      (HCN) channels between adult and aged dogs to identify possible mechanisms of 
      age-related atrial fibrillation. MATERIAL/METHODS: Corrected sinus node recovery 
      time (SNRTc) and effective refractory period (ERP) of the atrium and pulmonary 
      veins were measured in 10 adult (3-6 years old) and 10 aged dogs (>9 years old). 
      Expression levels of HCN2 and HCN4 channel mRNAs and proteins were measured in 
      the sinoatrial node, atrium, and pulmonary veins by real-time PCR and Western 
      blotting. RESULTS: Aged dogs exhibited a higher induction rate of atrial 
      fibrillation (AF) in response to electrical stimulation, longer AF duration after 
      induction, longer SNRTc, longer right atrial effective refractory period (AERP), 
      shorter left AERP, and increased AERP dispersion compared to adults. Expression 
      levels of HCN2 and HCN4 channel mRNAs and proteins were lower in the sinoatrial 
      node but higher in the atrium and pulmonary veins of aged dogs. CONCLUSIONS: 
      Changes in atrial electrophysiological indicators in aged dogs revealed 
      sinoatrial node dysfunction. There was a reversal in the local tissue 
      distribution of HCN2 and HCN4 channel mRNA and protein, a decrease in sinoatrial 
      node expression, and increase in atrial and pulmonary vein expression with age. 
      Changes in atrial electrophysiological characteristics and regional HCN channel 
      expression patterns were associated with the onset and maintenance of age-related 
      atrial fibrillation.
FAU - Li, Yao-Dong
AU  - Li YD
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Hong, Yi-Fan
AU  - Hong YF
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Zhou, Xian-Hui
AU  - Zhou XH
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Ji, Yu-Tong
AU  - Ji YT
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Li, Hong-Liang
AU  - Li HL
AD  - Heart Rhythm Institute and Endocrinology, University of Oklahoma Health Sciences 
      Center, Oklahoma City, OK, USA.
FAU - Hu, Guo-Jun
AU  - Hu GJ
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Li, Jin-Xin
AU  - Li JX
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Sun, Lin
AU  - Sun L
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Zhang, Jiang-Hua
AU  - Zhang JH
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Xin, Qiang
AU  - Xin Q
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Yusufuaji, Yueerguli
AU  - Yusufuaji Y
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Xiong, Jian
AU  - Xiong J
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
FAU - Tang, Bao-Peng
AU  - Tang BP
AD  - Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, 
      Urumqi, Xinjiang, China (mainland).
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141114
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Action Potentials
MH  - Aging/*metabolism
MH  - Animals
MH  - Atrial Fibrillation/*genetics/*physiopathology
MH  - Dogs
MH  - Gene Expression Regulation
MH  - Heart Atria/physiopathology
MH  - Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels/*genetics/metabolism
MH  - Pulmonary Veins/physiopathology
MH  - RNA, Messenger/genetics/metabolism
MH  - Refractory Period, Electrophysiological
PMC - PMC4242900
EDAT- 2014/11/19 06:00
MHDA- 2015/06/30 06:00
PMCR- 2014/11/14
CRDT- 2014/11/19 06:00
PHST- 2014/11/19 06:00 [entrez]
PHST- 2014/11/19 06:00 [pubmed]
PHST- 2015/06/30 06:00 [medline]
PHST- 2014/11/14 00:00 [pmc-release]
AID - 892505 [pii]
AID - 10.12659/MSM.892505 [doi]
PST - epublish
SO  - Med Sci Monit. 2014 Nov 14;20:2292-7. doi: 10.12659/MSM.892505.