PMID- 2540500
OWN - NLM
STAT- MEDLINE
DCOM- 19890608
LR  - 20190818
IS  - 0031-8655 (Print)
IS  - 0031-8655 (Linking)
VI  - 49
IP  - 2
DP  - 1989 Feb
TI  - Mechanisms for luminol-augmented chemiluminescence from neutrophils induced by 
      leukotriene B4 and N-formyl-methionyl-leucyl-phenylalanine.
PG  - 217-23
AB  - Neutrophils stimulated with formyl-methionyl-leucyl-phenylalanine (fMLP) or 
      leukotriene B4 (LTB4) generated kinetically distinctive luminol augmented 
      chemiluminescence (LCL). Inhibitors of .O2- [superoxide-dismutase (SOD) or 
      tiron], H2O2 (catalase), myeloperoxidase, MPO, (NaN3), HOCl (taurine) and .OH 
      (mannitol) hampered LCL dose-dependently with similar characteristics for both 
      stimuli. In cell free systems it was found that .O2- (generated in the 
      xanthine/xanthine-oxidase reaction) or H2O2 produced LCL. Superoxide dismutase 
      inhibited .O2- -induced LCL dose dependently. The MPO + H2O2 system, which 
      generated more pronounced LCL than either component alone, was inhibited by 
      catalase and taurine but not by SOD. When neutrophils, treated with luminol, but 
      where extracellular luminol had been removed, were stimulated with fMLP or LTB4, 
      they produced less than 2% of the LCL where luminol was present in the medium. 
      When neutrophil LCL and superoxide formation by the cytochrome C method were 
      assessed in parallel experiments, in all instances the peak LCL response 
      coincided with the linear phase in that response. Thus, LCL, induced by LTB4 and 
      the corresponding fMLP peak, are extracellular events with similar chemical 
      backgrounds, closely related to generation of reactive oxygen species. 
      Consequently, the kinetical differences in LCL between fMLP and LTB4 suggest that 
      LTB4, by yet unknown mechanisms, activates the NADPH oxidase more rapidly than 
      fMLP.
FAU - Gyllenhammar, H
AU  - Gyllenhammar H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Photochem Photobiol
JT  - Photochemistry and photobiology
JID - 0376425
RN  - 0 (Pyridazines)
RN  - 11062-77-4 (Superoxides)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 5EXP385Q4F (Luminol)
SB  - IM
MH  - Drug Synergism
MH  - Humans
MH  - In Vitro Techniques
MH  - Leukotriene B4/*pharmacology
MH  - Luminescence
MH  - Luminol/*pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/drug effects/*physiology
MH  - Pyridazines/*pharmacology
MH  - Superoxides/*blood
EDAT- 1989/02/01 00:00
MHDA- 1989/02/01 00:01
CRDT- 1989/02/01 00:00
PHST- 1989/02/01 00:00 [pubmed]
PHST- 1989/02/01 00:01 [medline]
PHST- 1989/02/01 00:00 [entrez]
AID - 10.1111/j.1751-1097.1989.tb04099.x [doi]
PST - ppublish
SO  - Photochem Photobiol. 1989 Feb;49(2):217-23. doi: 
      10.1111/j.1751-1097.1989.tb04099.x.