PMID- 25405216
OWN - NLM
STAT- MEDLINE
DCOM- 20151116
LR  - 20220321
IS  - 1537-744X (Electronic)
IS  - 2356-6140 (Print)
IS  - 1537-744X (Linking)
VI  - 2014
DP  - 2014
TI  - The role of Hibiscus sabdariffa L. (Roselle) in maintenance of ex vivo murine 
      bone marrow-derived hematopoietic stem cells.
PG  - 258192
LID - 10.1155/2014/258192 [doi]
LID - 258192
AB  - Hematopoietic stem cells- (HSCs-) based therapy requires ex vivo expansion of 
      HSCs prior to therapeutic use. However, ex vivo culture was reported to promote 
      excessive production of reactive oxygen species (ROS), exposing HSCs to oxidative 
      damage. Efforts to overcome this limitation include the use of antioxidants. In 
      this study, the role of Hibiscus sabdariffa L. (Roselle) in maintenance of 
      cultured murine bone marrow-derived HSCs was investigated. Aqueous extract of 
      Roselle was added at varying concentrations (0-1000 ng/mL) for 24 hours to the 
      freshly isolated murine bone marrow cells (BMCs) cultures. Effects of Roselle on 
      cell viability, reactive oxygen species (ROS) production, glutathione (GSH) 
      level, superoxide dismutase (SOD) activity, and DNA damage were investigated. 
      Roselle enhanced the survival (P < 0.05) of BMCs at 500 and 1000 ng/mL, increased 
      survival of Sca-1(+) cells (HSCs) at 500 ng/mL, and maintained HSCs phenotype as 
      shown from nonremarkable changes of surface marker antigen (Sca-1) expression in 
      all experimental groups. Roselle increased (P < 0.05) the GSH level and SOD 
      activity but the level of reactive oxygen species (ROS) was unaffected. Moreover, 
      Roselle showed significant cellular genoprotective potency against H2O2-induced 
      DNA damage. Conclusively, Roselle shows novel property as potential supplement 
      and genoprotectant against oxidative damage to cultured HSCs.
FAU - Abdul Hamid, Zariyantey
AU  - Abdul Hamid Z
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
FAU - Lin Lin, Winnie Hii
AU  - Lin Lin WH
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
FAU - Abdalla, Basma Jibril
AU  - Abdalla BJ
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
FAU - Bee Yuen, Ong
AU  - Bee Yuen O
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
FAU - Latif, Elda Surhaida
AU  - Latif ES
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
FAU - Mohamed, Jamaludin
AU  - Mohamed J
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
FAU - Rajab, Nor Fadilah
AU  - Rajab NF
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
FAU - Paik Wah, Chow
AU  - Paik Wah C
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
FAU - Wak Harto, Muhd Khairul Akmal
AU  - Wak Harto MK
AUID- ORCID: 0000-0002-8538-6592
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
FAU - Budin, Siti Balkis
AU  - Budin SB
AD  - Program of Biomedical Science, School of Diagnostic and Applied Health Sciences, 
      Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Abdul Muda 
      Aziz, 50300 Kuala Lumpur, Malaysia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141021
PL  - United States
TA  - ScientificWorldJournal
JT  - TheScientificWorldJournal
JID - 101131163
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/drug effects/physiology
MH  - Cell Culture Techniques/*methods
MH  - Cell Survival/drug effects/physiology
MH  - Hematopoietic Stem Cells/*drug effects/*physiology
MH  - *Hibiscus
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Reactive Oxygen Species/metabolism
PMC - PMC4227436
EDAT- 2014/11/19 06:00
MHDA- 2015/11/17 06:00
PMCR- 2014/10/21
CRDT- 2014/11/19 06:00
PHST- 2014/06/11 00:00 [received]
PHST- 2014/09/25 00:00 [accepted]
PHST- 2014/11/19 06:00 [entrez]
PHST- 2014/11/19 06:00 [pubmed]
PHST- 2015/11/17 06:00 [medline]
PHST- 2014/10/21 00:00 [pmc-release]
AID - 10.1155/2014/258192 [doi]
PST - ppublish
SO  - ScientificWorldJournal. 2014;2014:258192. doi: 10.1155/2014/258192. Epub 2014 Oct 
      21.