PMID- 25405642
OWN - NLM
STAT- MEDLINE
DCOM- 20151015
LR  - 20141208
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 11
IP  - 3
DP  - 2015 Mar
TI  - Endoplasmic reticulum stress protects human thyroid carcinoma cell lines against 
      ionizing radiation-induced apoptosis.
PG  - 2341-7
LID - 10.3892/mmr.2014.2956 [doi]
AB  - Radiotherapy is one of the most effective forms of cancer treatment, used in the 
      treatment of a number of malignant tumors. However, the resistance of tumor cells 
      to ionizing radiation remains a major therapeutic problem and the critical 
      mechanisms determining radiation resistance are poorly defined. In the present 
      study, a cellular endoplasmic reticulum (ER) stress microenvironment was 
      established through the pretreatment of cultured thyroid cancer cells with 
      tunicamycin (TM) and thapsigargin (TG), in order to mimic the ER stress response 
      in a tumor microenvironment. This microenviroment was confirmed through the X‑box 
      binding protein 1 splice process, glucose‑regulated protein 78 kD and ER 
      degradation‑enhancing alpha‑mannosidase‑like mRNA expression. A clonogenic assay was 
      used to measure cancer cell resistance to 60Co‑gamma following TM pretreatment; in 
      addition, human C/EBP homologous protein (CHOP) mRNA expression was determined 
      and apoptosis assays were performed. The results showed that TM or TG 
      pretreatment inhibited CHOP expression and reduced the apoptotic rate of cells. 
      Furthermore, the results demonstrated that the induced ER stress response 
      rendered cancer cells more resistant to ionizing radiation‑induced apoptosis. 
      Therefore, the ER stress pathway may be a potential therapeutic target in order 
      to improve the clinical efficiency of radiotherapy.
FAU - Wu, Xin-Yu
AU  - Wu XY
AD  - Department of Nuclear Medicine, Henan Provincial People's Hospital and People's 
      Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China.
FAU - Fan, Rui-Tai
AU  - Fan RT
AD  - Department of Radiation Oncology, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, Henan 450052, P.R. China.
FAU - Yan, Xin-Hui
AU  - Yan XH
AD  - Department of Nuclear Medicine, Henan Provincial People's Hospital and People's 
      Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China.
FAU - Cui, Jing
AU  - Cui J
AD  - Department of Nuclear Medicine, Henan Provincial People's Hospital and People's 
      Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China.
FAU - Xu, Jun-Ling
AU  - Xu JL
AD  - Department of Nuclear Medicine, Henan Provincial People's Hospital and People's 
      Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China.
FAU - Gu, Hao
AU  - Gu H
AD  - Department of Radiation Oncology, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, Henan 450052, P.R. China.
FAU - Gao, Yong-Ju
AU  - Gao YJ
AD  - Department of Nuclear Medicine, Henan Provincial People's Hospital and People's 
      Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20141114
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
SB  - IM
MH  - *Apoptosis/radiation effects
MH  - Cell Line, Tumor
MH  - *Endoplasmic Reticulum Stress/radiation effects
MH  - Humans
MH  - *Radiation Tolerance
MH  - *Radiation, Ionizing
MH  - Thyroid Neoplasms/metabolism/*radiotherapy
EDAT- 2014/11/19 06:00
MHDA- 2015/10/16 06:00
CRDT- 2014/11/19 06:00
PHST- 2014/01/10 00:00 [received]
PHST- 2014/10/31 00:00 [accepted]
PHST- 2014/11/19 06:00 [entrez]
PHST- 2014/11/19 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
AID - 10.3892/mmr.2014.2956 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Mar;11(3):2341-7. doi: 10.3892/mmr.2014.2956. Epub 2014 Nov 14.