PMID- 25406855
OWN - NLM
STAT- MEDLINE
DCOM- 20160107
LR  - 20150407
IS  - 1537-6524 (Electronic)
IS  - 1537-6516 (Linking)
VI  - 25
IP  - 2
DP  - 2015 Feb
TI  - Effect of deoxycholic acid on Ca2+ movement, cell viability and apoptosis in 
      human gastric cancer cells.
PG  - 113-9
LID - 10.3109/15376516.2014.990597 [doi]
AB  - Deoxycholic acid (DOA) is one of the secondary bile acids used as a mild 
      detergent for the isolation of membrane associated proteins. This study examined 
      whether the secondary bile acid, DOA, altered Ca(2+) movement, cell viability and 
      apoptosis in SCM1 human gastric cancer cells. The Ca(2+)-sensitive fluorescent 
      dye fura-2 was used to measure [Ca(2+)]i. DOA-evoked [Ca(2+)]i rises 
      concentration dependently. The response was reduced by removing extracellular 
      Ca(2+). DOA-evoked Ca(2+) entry was inhibited by store-operated Ca(2+) channel 
      inhibitors (nifedipine, econazole and SKF96365), the protein kinase C (PKC) 
      activator phorbol 12-myristate 13 acetate (PMA) and the PKC inhibitor GF109203X. 
      In Ca(2+)-free medium, treatment with the endoplasmic reticulum Ca(2+) pump 
      inhibitor thapsigargin (TG) abolished DOA-evoked [Ca(2+)]i rises. Conversely, 
      treatment with DOA abolished TG-evoked [Ca(2+)]i rises. Inhibition of 
      phospholipase C with U73122 abolished DOA-evoked [Ca(2+)]i rises. At 100-500 muM, 
      DOA decreased cell viability, which was not changed by chelating cytosolic Ca(2+) 
      with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester 
      (BAPTA/AM). DOA between 100 and 300 muM also induced apoptosis. Collectively, in 
      SCM1 cells, DOA-induced [Ca(2+)]i rises by evoking phospholipase C-dependent 
      Ca(2+) release from the endoplasmic reticulum and Ca(2+) entry via store-operated 
      Ca(2+) channels. DOA also caused Ca(2+)-independent apoptosis.
FAU - Chien, Jau-Min
AU  - Chien JM
AD  - Department of Pediatrics, Ping Tung Christian Hospital , Ping Tung , Taiwan .
FAU - Chou, Chiang-Ting
AU  - Chou CT
FAU - Liang, Wei-Zhe
AU  - Liang WZ
FAU - Cheng, Jin-Shiung
AU  - Cheng JS
FAU - Chang, Hong-Tai
AU  - Chang HT
FAU - Tseng, Hui-Wen
AU  - Tseng HW
FAU - Kuo, Soong-Yu
AU  - Kuo SY
FAU - Kuo, Chun-Chi
AU  - Kuo CC
FAU - Chen, Fu-An
AU  - Chen FA
FAU - Shieh, Pochuen
AU  - Shieh P
FAU - Ho, Chin-Man
AU  - Ho CM
FAU - Lin, Jia-Rong
AU  - Lin JR
FAU - Kuo, Daih-Huang
AU  - Kuo DH
FAU - Jan, Chung-Ren
AU  - Jan CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150224
PL  - England
TA  - Toxicol Mech Methods
JT  - Toxicology mechanisms and methods
JID - 101134521
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Chelating Agents)
RN  - 0 (Enzyme Activators)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 005990WHZZ (Deoxycholic Acid)
RN  - 105344-37-4 (fura-2-am)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Calcium/*metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Chelating Agents/pharmacology
MH  - Calcium Signaling/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Survival/*drug effects
MH  - Deoxycholic Acid/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Activation
MH  - Enzyme Activators/pharmacology
MH  - Fura-2/analogs & derivatives/metabolism
MH  - Humans
MH  - Microscopy, Fluorescence
MH  - Protein Kinase C/antagonists & inhibitors/metabolism
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases/antagonists & 
      inhibitors/metabolism
MH  - Stomach Neoplasms/*drug therapy/metabolism/pathology
MH  - Time Factors
OTO - NOTNLM
OT  - Apoptosis
OT  - Ca2+
OT  - deoxycholic acid
OT  - endoplasmic reticulum
OT  - gastric cancer cells
EDAT- 2014/11/20 06:00
MHDA- 2016/01/08 06:00
CRDT- 2014/11/20 06:00
PHST- 2014/11/20 06:00 [entrez]
PHST- 2014/11/20 06:00 [pubmed]
PHST- 2016/01/08 06:00 [medline]
AID - 10.3109/15376516.2014.990597 [doi]
PST - ppublish
SO  - Toxicol Mech Methods. 2015 Feb;25(2):113-9. doi: 10.3109/15376516.2014.990597. 
      Epub 2015 Feb 24.