PMID- 25408532
OWN - NLM
STAT- MEDLINE
DCOM- 20150903
LR  - 20181202
IS  - 1872-8081 (Electronic)
IS  - 0951-6433 (Linking)
VI  - 40
IP  - 6
DP  - 2014 Nov-Dec
TI  - Biological parameters influencing the human umbilical cord-derived mesenchymal 
      stem cells' response to retinoic acid.
PG  - 624-35
LID - 10.1002/biof.1192 [doi]
AB  - Human umbilical cord-derived mesenchymal stem cells (HUCMSCs) are multipotent 
      fetal stem cells that differentiate into various cell lineages. In recent years, 
      they have gained attention for therapeutic applications but very little is known 
      about their sensitivity to chemical agents such as widely used retinoic acid 
      (RA). As a morphogen inducing differentiation of mesenchymal stem cells, RA has 
      for a long time been known to be a potent teratogen promoting craniofacial and 
      limb abnormality in vertebrate embryos. Here, using MTT assay and EB/AO staining 
      as well as TUNEL assay we show that RA in a concentration-dependent manner 
      induces apoptosis through upregulating Caspase expression and increasing Bax/Bcl2 
      ratio. Moreover, different biological parameters such as initial time seeding, 
      cell density, passage number and duration of RA treatment play a major role in 
      HUCMSCs cytotoxic response to this agent.
CI  - (c) 2014 International Union of Biochemistry and Molecular Biology.
FAU - Saraee, Farnoosh
AU  - Saraee F
AD  - Research Laboratory for Embryology and Stem Cells, Department of Anatomical 
      Sciences and Pathology, Faculty of Medicine, Ardabil University of Medical 
      Sciences, Ardabil, Iran; Department of Animal Sciences, School of Life Sciences, 
      University of Kharazmi, Tehran, Iran.
FAU - Sagha, Mohsen
AU  - Sagha M
FAU - Mohseni Kouchesfehani, Homa
AU  - Mohseni Kouchesfehani H
FAU - Abdanipour, Alireza
AU  - Abdanipour A
FAU - Maleki, Masoud
AU  - Maleki M
FAU - Nikougoftar, Mahin
AU  - Nikougoftar M
LA  - eng
PT  - Journal Article
DEP - 20141119
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Antigens, CD)
RN  - 0 (BAX protein, human)
RN  - 0 (BCL2 protein, human)
RN  - 0 (Cytotoxins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 5688UTC01R (Tretinoin)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Antigens, CD/genetics/metabolism
MH  - Apoptosis/drug effects
MH  - Caspases/genetics/metabolism
MH  - Cell Count
MH  - Cell Differentiation/drug effects
MH  - Cytotoxins/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Fetal Blood/cytology/*drug effects/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*drug effects/metabolism
MH  - Primary Cell Culture
MH  - Proto-Oncogene Proteins c-bcl-2/antagonists & inhibitors/genetics/metabolism
MH  - Signal Transduction
MH  - Tretinoin/*toxicity
MH  - bcl-2-Associated X Protein/agonists/genetics/metabolism
OTO - NOTNLM
OT  - apoptosis
OT  - cytotoxicity
OT  - human umbilical cord-derived mesenchymal stem cells
OT  - retinoic acid
EDAT- 2014/11/20 06:00
MHDA- 2015/09/04 06:00
CRDT- 2014/11/20 06:00
PHST- 2014/07/21 00:00 [received]
PHST- 2014/10/16 00:00 [revised]
PHST- 2014/11/02 00:00 [accepted]
PHST- 2014/11/20 06:00 [entrez]
PHST- 2014/11/20 06:00 [pubmed]
PHST- 2015/09/04 06:00 [medline]
AID - 10.1002/biof.1192 [doi]
PST - ppublish
SO  - Biofactors. 2014 Nov-Dec;40(6):624-35. doi: 10.1002/biof.1192. Epub 2014 Nov 19.