PMID- 25408545
OWN - NLM
STAT- MEDLINE
DCOM- 20150507
LR  - 20200411
IS  - 1096-9896 (Electronic)
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 235
IP  - 5
DP  - 2015 Apr
TI  - BDNF repairs podocyte damage by microRNA-mediated increase of actin 
      polymerization.
PG  - 731-44
LID - 10.1002/path.4484 [doi]
AB  - Idiopathic focal segmental glomerulosclerosis (FSGS) is a progressive and 
      proteinuric kidney disease that starts with podocyte injury. Podocytes cover the 
      external side of the glomerular capillary by a complex web of primary and 
      secondary ramifications. Similar to dendritic spines of neuronal cells, podocyte 
      processes rely on a dynamic actin-based cytoskeletal architecture to maintain 
      shape and function. Brain-derived neurotrophic factor (BDNF) is a pleiotropic 
      neurotrophin that binds to the tropomyosin-related kinase B receptor (TrkB) and 
      has crucial roles in neuron maturation, survival, and activity. In neuronal 
      cultures, exogenously added BDNF increases the number and size of dendritic 
      spines. In animal models, BDNF administration is beneficial in both central and 
      peripheral nervous system disorders. Here we show that BDNF has a TrkB-dependent 
      trophic activity on podocyte cell processes; by affecting microRNA-134 and 
      microRNA-132 signalling, BDNF up-regulates Limk1 translation and phosphorylation, 
      and increases cofilin phosphorylation, which results in actin polymerization. 
      Importantly, BDNF effectively repairs podocyte damage in vitro, and contrasts 
      proteinuria and glomerular lesions in in vivo models of FSGS, opening a potential 
      new perspective to the treatment of podocyte disorders.
CI  - Copyright (c) 2014 Pathological Society of Great Britain and Ireland. Published by 
      John Wiley & Sons, Ltd.
FAU - Li, Min
AU  - Li M
AD  - Renal Research Laboratory, Fondazione D'Amico per la Ricerca sulle Malattie 
      Renali & Fondazione IRCCS Ca', Granda Ospedale Maggiore Policlinico, Milano, 
      Italy.
FAU - Armelloni, Silvia
AU  - Armelloni S
FAU - Zennaro, Cristina
AU  - Zennaro C
FAU - Wei, Changli
AU  - Wei C
FAU - Corbelli, Alessandro
AU  - Corbelli A
FAU - Ikehata, Masami
AU  - Ikehata M
FAU - Berra, Silvia
AU  - Berra S
FAU - Giardino, Laura
AU  - Giardino L
FAU - Mattinzoli, Deborah
AU  - Mattinzoli D
FAU - Watanabe, Shojiro
AU  - Watanabe S
FAU - Agostoni, Carlo
AU  - Agostoni C
FAU - Edefonti, Alberto
AU  - Edefonti A
FAU - Reiser, Jochen
AU  - Reiser J
FAU - Messa, Piergiorgio
AU  - Messa P
FAU - Rastaldi, Maria Pia
AU  - Rastaldi MP
LA  - eng
GR  - R01 DK073495/DK/NIDDK NIH HHS/United States
GR  - R01 DK089394/DK/NIDDK NIH HHS/United States
GR  - DK073495/DK/NIDDK NIH HHS/United States
GR  - DK089394/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150107
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Actin Depolymerizing Factors)
RN  - 0 (Actins)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN132 microRNA, human)
RN  - 0 (MIRN132 microRNA, mouse)
RN  - 0 (MIRN134 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn134 microRNA, mouse)
RN  - 0 (Recombinant Proteins)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.1 (Lim Kinases)
SB  - IM
MH  - 3T3 Cells
MH  - Actin Cytoskeleton/*drug effects/metabolism/pathology
MH  - Actin Depolymerizing Factors/metabolism
MH  - Actins/*metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Disease Models, Animal
MH  - Gene Expression Regulation
MH  - Glomerulosclerosis, Focal Segmental/chemically 
      induced/genetics/metabolism/pathology/*prevention & control
MH  - Larva/drug effects/metabolism
MH  - Lim Kinases/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/*metabolism
MH  - Phosphorylation
MH  - Podocytes/*drug effects/metabolism/pathology
MH  - Polymerization
MH  - Proteinuria/metabolism/prevention & control
MH  - RNA Interference
MH  - Receptor, trkB/metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Signal Transduction/drug effects
MH  - Time Factors
MH  - Transfection
MH  - Zebrafish
PMC - PMC4356648
MID - NIHMS644407
OTO - NOTNLM
OT  - actin cytoskeleton
OT  - adriamycin nephropathy
OT  - brain-derived neurotrophic factor
OT  - podocyte
EDAT- 2014/11/20 06:00
MHDA- 2015/05/08 06:00
PMCR- 2016/04/01
CRDT- 2014/11/20 06:00
PHST- 2014/05/28 00:00 [received]
PHST- 2014/10/17 00:00 [revised]
PHST- 2014/11/11 00:00 [accepted]
PHST- 2014/11/20 06:00 [entrez]
PHST- 2014/11/20 06:00 [pubmed]
PHST- 2015/05/08 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - 10.1002/path.4484 [doi]
PST - ppublish
SO  - J Pathol. 2015 Apr;235(5):731-44. doi: 10.1002/path.4484. Epub 2015 Jan 7.