PMID- 25408547
OWN - NLM
STAT- MEDLINE
DCOM- 20151125
LR  - 20181113
IS  - 1476-3524 (Electronic)
IS  - 1029-8428 (Print)
IS  - 1029-8428 (Linking)
VI  - 27
IP  - 3
DP  - 2015 Apr
TI  - Withdrawal from cocaine self-administration and yoked cocaine delivery 
      dysregulates glutamatergic mGlu5 and NMDA receptors in the rat brain.
PG  - 246-58
LID - 10.1007/s12640-014-9502-z [doi]
AB  - In human addicts and in animal models, chronic cocaine use leads to numerous 
      alterations in glutamatergic transmission, including its receptors. The present 
      study focused on metabotropic glutamatergic receptors type 5 (mGluR(5)) and 
      N-methyl-D-aspartate receptor subunits (NMDAR: GluN1, GluN2A, GluN2B) proteins 
      during cocaine self-administration and after 10-day of extinction training in 
      rats. To discriminate the contingent from the non-contingent cocaine delivery, we 
      employed the "yoked"-triad control procedure. Protein expression in rat 
      prefrontal cortex, nucleus accumbens, hippocampus, and dorsal striatum was 
      determined. We also examined the Homer1b/c protein, a member of the postsynaptic 
      density protein family that links NMDAR to mGluR(5). Our results revealed that 
      cocaine self-administration selectively increased GluN1 and GluN2A subunit in the 
      rat hippocampus and dorsal striatum, respectively, while mGluR(5) protein 
      expression was similarly increased in the dorsal striatum of both experimental 
      groups. Withdrawal from both contingent and non-contingent cocaine delivery 
      induced parallel increases in prefrontal cortical GluN2A protein expression, 
      hippocampal mGluR(5), and GluN1 protein expression as well as in accumbal GluN1 
      subunit expression, while the mGluR(5) expression was reduced in the prefrontal 
      cortex. Extinction training in animals with a history of cocaine 
      self-administration resulted in an elevation of the hippocampal GluN2A/GluN2B 
      subunits and accumbal mGluR(5), and in a 50 % decrease of mGluR(5) protein 
      expression in the dorsal striatum. The latter reduction was associated with 
      Homer1b/1c protein level decrease. Our results showed that both contingent and 
      non-contingent cocaine administration produces numerous, brain region specific, 
      alterations in the mGluR(5), NMDA, and Homer1b/1c protein expression which are 
      dependent on the modality of cocaine administration.
FAU - Pomierny-Chamiolo, Lucyna
AU  - Pomierny-Chamiolo L
AD  - Department of Toxicology, Department of Biochemical Toxicology, Faculty of 
      Pharmacy, Jagiellonian University, Medical College, Medyczna 9, 30-688, Krakow, 
      Poland, lpomiern@cm-uj.krakow.pl.
FAU - Miszkiel, Joanna
AU  - Miszkiel J
FAU - Frankowska, Malgorzata
AU  - Frankowska M
FAU - Pomierny, Bartosz
AU  - Pomierny B
FAU - Niedzielska, Ewa
AU  - Niedzielska E
FAU - Smaga, Irena
AU  - Smaga I
FAU - Fumagalli, Fabio
AU  - Fumagalli F
FAU - Filip, Malgorzata
AU  - Filip M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141119
PL  - United States
TA  - Neurotox Res
JT  - Neurotoxicity research
JID - 100929017
RN  - 0 (Carrier Proteins)
RN  - 0 (Grm5 protein, rat)
RN  - 0 (Homer Scaffolding Proteins)
RN  - 0 (Protein Subunits)
RN  - 0 (Receptor, Metabotropic Glutamate 5)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - I5Y540LHVR (Cocaine)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/*metabolism
MH  - Carrier Proteins/metabolism
MH  - Cocaine/administration & dosage/*toxicity
MH  - Extinction, Psychological/drug effects
MH  - Homer Scaffolding Proteins
MH  - Male
MH  - Protein Subunits/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Metabotropic Glutamate 5/*metabolism
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Self Administration
MH  - Substance Withdrawal Syndrome/*metabolism
PMC - PMC4353866
EDAT- 2014/11/20 06:00
MHDA- 2015/12/15 06:00
PMCR- 2014/11/19
CRDT- 2014/11/20 06:00
PHST- 2014/08/04 00:00 [received]
PHST- 2014/11/06 00:00 [accepted]
PHST- 2014/10/06 00:00 [revised]
PHST- 2014/11/20 06:00 [entrez]
PHST- 2014/11/20 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2014/11/19 00:00 [pmc-release]
AID - 9502 [pii]
AID - 10.1007/s12640-014-9502-z [doi]
PST - ppublish
SO  - Neurotox Res. 2015 Apr;27(3):246-58. doi: 10.1007/s12640-014-9502-z. Epub 2014 
      Nov 19.