PMID- 25410064
OWN - NLM
STAT- MEDLINE
DCOM- 20150212
LR  - 20141121
IS  - 1011-601X (Print)
IS  - 1011-601X (Linking)
VI  - 27
IP  - 6 Suppl
DP  - 2014 Nov
TI  - Screening for potential -alpha -Glucosidase inhibitors in Coptis chinensis franch 
      extract using ultrafiltration LC-ESI-MSn.
PG  - 2007-12
AB  - Naturally existing -alpha -glucosidase inhibitors from traditional herbal medicines 
      have attracted considerable interest to treat type 2 diabetes mellitus (T2DM). 
      Hundreds of herbs have been reported to have the potential to inhibit -alpha 
      -glucosidase. However, most common methods to examine the inhibitors of -alpha 
      -glucosidase are usually time-consuming. In the current study, the screening of 
      -alpha -glucosidase ligands from Coptis chinensis Franch extract was undertaken by 
      ultrafiltration liquid chromatography coupled to electrospray ionization tandem 
      mass spectrometry (ultrafiltration LC-ESI-MS(n)). Resultantly, the enzyme 
      inhibition studies showed that Coptis chinensis Franch extract carries the 
      strongest -alpha -glucosidase inhibitory activity among the five kinds of Chinese 
      herbal extracts. Subsequently, five compounds that could bind to -alpha -glucosidase 
      in the Coptis chinensis Franch extract were found using ultrafiltration liquid 
      chromatography, and their structures were identified by ESI-MS(n) to be 
      coptisine, epiberberine, jatrorrhizine, berberine, palmatine. Cumulatively, these 
      results were anticipated to be encouraging for applying the Coptis chinensis 
      Franch extracts as efficient anti-diabetic drug candidates.
FAU - Zhou, Hui
AU  - Zhou H
AD  - Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, 
      P.R. China / Changchun Center of Mass Spectrometry, Changchun Institute of 
      Applied Chemistry, Chinese Academy of Sciences, Changchun, P.R. China.
FAU - Jiang, Tao
AU  - Jiang T
AD  - Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, 
      P.R. China.
FAU - Wang, Zhengyang
AU  - Wang Z
AD  - Institute of Theoretical Chemistry, Jilin University, Changchun, P.R. China.
FAU - Ren, Shen
AU  - Ren S
AD  - College of Traditional Chinese Medicine, Jilin Agricultural University, 
      Changchun, P.R. China.
FAU - Zhao, Xi
AU  - Zhao X
AD  - Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, 
      P.R. China.
FAU - Wu, Wei
AU  - Wu W
AD  - Department of Biomedical Sciences, Texas Tech University Health Sciences Center, 
      School of Pharmacy, Amarillo, TX USA.
FAU - Jiang, Lianhai
AU  - Jiang L
AD  - Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, 
      P.R. China.
FAU - Liu, Zhiqiang
AU  - Liu Z
AD  - Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, 
      Chinese Academy of Sciences, Changchun, P.R. China.
FAU - Teng, Lirong
AU  - Teng L
AD  - Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, 
      P.R. China.
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - Pak J Pharm Sci
JT  - Pakistan journal of pharmaceutical sciences
JID - 9426356
RN  - 0 (Glycoside Hydrolase Inhibitors)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Coptis/*chemistry
MH  - Glycoside Hydrolase Inhibitors/*analysis
MH  - Plant Extracts/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Ultrafiltration
EDAT- 2014/11/21 06:00
MHDA- 2015/02/13 06:00
CRDT- 2014/11/21 06:00
PHST- 2014/11/21 06:00 [entrez]
PHST- 2014/11/21 06:00 [pubmed]
PHST- 2015/02/13 06:00 [medline]
PST - ppublish
SO  - Pak J Pharm Sci. 2014 Nov;27(6 Suppl):2007-12.