PMID- 25411013
OWN - NLM
STAT- MEDLINE
DCOM- 20150706
LR  - 20220409
IS  - 1743-6109 (Electronic)
IS  - 1743-6095 (Linking)
VI  - 12
IP  - 3
DP  - 2015 Mar
TI  - Effect of chronic administration of PDE5 combined with glycemic control on 
      erectile function in streptozotocin-induced diabetic rats.
PG  - 600-10
LID - 10.1111/jsm.12752 [doi]
AB  - INTRODUCTION: Chronic treatment with phosphodiesterase type 5 inhibitors (PDE5) 
      is effective in an animal model of diabetes-induced erectile dysfunction (DMED). 
      In addition, recent research indicates that glycemic control can restore DMED. 
      AIMS: We evaluated the effect of chronic administration of PDE5 combined with 
      glycemic control on DMED. METHODS: Sprague-Dawley rats (8 weeks old) were divided 
      into five groups (n = 10 each): normal control (C), diabetes (DM), DM treated 
      with insulin (DM-I), DM treated with PDE5 (DM-P), and DM treated with insulin and 
      PDE5 (DM-I + P). Rats in the diabetic groups received an injection of 
      streptozotocin (45 mg/kg). After 10 weeks of induced diabetes, the DM-I group was 
      treated with a daily injection of neutral protamine Hagedorn, and the DM-P group 
      was treated with a daily dosage of 20 mg/kg PDE5 (DA-8159) for 4 weeks. The 
      DM-I + P group was treated with both treatments simultaneously. After 14 weeks of 
      induced diabetes, an evaluation of erectile function and histological and 
      biochemical markers of corporal tissue was performed. MAIN OUTCOME MEASURES: 
      Erectile function and histological and biochemical markers in corporal tissue. 
      RESULTS: Rats in the DM group showed markedly lower erectile parameters than 
      those in the C group, whereas rats in the DM-I and DM-P groups showed 
      intermediate erectile function between the DM and C groups. Rats in the DM-I + P 
      group showed restored erectile function, comparable with group C. A comparison of 
      apoptotic index, expression of the endothelial marker, and phosphorylation of 
      endothelial nitric oxide synthase and Akt displayed a similar pattern with the 
      results from cavernosometry (DM < DM-I = DM-P < DM-I + P = C, P < 0.05). The 
      distribution of phosphorylated myosin phosphatase target subunit 1 was in the 
      reverse order. CONCLUSIONS: Chronic administration of PDE5 or glycemic control 
      with insulin resulted in restoration of overt DMED. The combination of both 
      treatments was superior to monotherapy with insulin or PDE5.
CI  - (c) 2014 International Society for Sexual Medicine.
FAU - Choi, Woo Suk
AU  - Choi WS
AD  - Department of Urology, Konkuk University Hospital, Seoul, Korea.
FAU - Kwon, Oh Seong
AU  - Kwon OS
FAU - Cho, Sung Yong
AU  - Cho SY
FAU - Paick, Jae-Seung
AU  - Paick JS
FAU - Kim, Soo Woong
AU  - Kim SW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141120
PL  - Netherlands
TA  - J Sex Med
JT  - The journal of sexual medicine
JID - 101230693
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (Phosphodiesterase 5 Inhibitors)
RN  - 0 (Pyrimidines)
RN  - 0 (Sulfonamides)
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - L5IB4XLY36 (udenafil)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - Diabetes Mellitus, Experimental/complications/*drug therapy/physiopathology
MH  - Drug Therapy, Combination
MH  - Erectile Dysfunction/*drug therapy/etiology/physiopathology
MH  - Hypoglycemic Agents/*therapeutic use
MH  - Insulin/*therapeutic use
MH  - Male
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Penile Erection/drug effects
MH  - Phosphodiesterase 5 Inhibitors/*therapeutic use
MH  - Pyrimidines/*therapeutic use
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Streptozocin
MH  - Sulfonamides/*therapeutic use
OTO - NOTNLM
OT  - Animal Model of Diabetes
OT  - Diabetes Mellitus
OT  - Erectile Dysfunction
EDAT- 2014/11/21 06:00
MHDA- 2015/07/07 06:00
CRDT- 2014/11/21 06:00
PHST- 2014/11/21 06:00 [entrez]
PHST- 2014/11/21 06:00 [pubmed]
PHST- 2015/07/07 06:00 [medline]
AID - S1743-6095(15)30947-4 [pii]
AID - 10.1111/jsm.12752 [doi]
PST - ppublish
SO  - J Sex Med. 2015 Mar;12(3):600-10. doi: 10.1111/jsm.12752. Epub 2014 Nov 20.